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Bioscience Methods 2012, Vol.3, No.4, 27
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combination of BA and IAA could also induce buds
but the number of the induced shoots of each explant
was less than that of the combination of ZT and IAA.
It is interesting that the combination of BA and NAA
is better for the callus growth than other combinations
and the callus tissues were 2
-
fold larger more than
others (Figure 1B). However, the callus tissues was
obviously green, and tissue structure was swell and
loose, but there was no bud forming any more, which
indicated that the combination of NAA and BA should
facilitate to callus tissue growth but not to bud
formation. The results showed that the medium of MS
+ ZT of 2 mg/L+IAA 0.1 mg/L, had the highest
tomato bud induced rate, while the best medium for
inducing tomato callus tissues was MS + BA 1.0 mg/L,
+ NAA 1.0 mg/L .
1.3 The effects of the different types of explants on
bud differentiation
Steriled tomato cotyledon, leaf, stem and root
segments were placed on the selected best bud
inducing medium; after 4 day culture, it could see
cotyledons and leaves expanding significantly,
thickening, color fading but lighting; while stem
segments became thickening, elongating, going to
yellowish and a little bit villous aerial root appearing.
Table 2 Effects of different hormone combination on induction of adventitious buds
Hormone combination (mg/L) No. of
explants
No. of callus
induction
Ratio of callus
induction (%)
No. of buds
induction
Ratio of buds
induction (%)
Sprouting
number
BA
IAA
NAA ZT
1.0
1.0
1.0
2.0
2.0
2.0
3.0
3.0
3.0
0.7
0.7
0.7
1.0
1.0
1.0
1.3
1.3
1.3
-
-
-
-
-
-
-
-
-
-
-
0.1
0.2
0.3
0.1
0.2
0.3
0.1
0.2
0.3
-
-
-
-
-
-
-
-
-
0
0.05
0.10
0.20
0
0.05
0.10
0.20
0
0.05
0.10
0.20
-
-
-
-
-
-
-
-
-
0.7
1.0
1.3
0.7
1.0
1.3
0.7
1.0
1.3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1.5
1.5
1.5
1.5
2.0
2.0
2.0
2.0
2.5
2.5
2.5
2.5
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
20
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
100.0
20
20
20
20
20
20
20
3.3
2.4
0
0
0
0
0
0
0
0
0
20
20
20
20
20
20
20
20
20
20
10.5
3.6
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
19.6±0.37dD
15.0±0.25eE
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
0.0
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
100.0±0.00aA
75.4±0.43bB
60.2±0.11cC
2~3
2~3
3~4
2~3
2~3
2~3
2~3
0~1
0~1
0
0
0
0
0
0
0
0
0
2~3
3~4
4~5
4~5
3~4
5~6
5~6
2~3
2~3
3~4
2~3
1~2