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Bioscience Methods 2012, Vol.3, No.3, 21
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Figure 2 Adventitious buds propagating
Note: A: The buds on the medium with treatment 2 after 30 d;
B: The buds on the medium with treatment 6 after 30 d
which will induce the shoot to a complete plant (Table
5). Among these mediums, the result of the 0.1 mg/L
NAA MS medium with the 65% rooting rate was
better. However, the length of adventitious buds
needed to be longer than 1.5 cm in order to obtain
better root, whilst the length of adventitious buds was
shorter than 1.5 cm, the roots grew fewer and slower,
so did the whole plantlet (Figure 3).
2 Discussion
With the increasing of people living standard,
natural extracts and essential oils of
R. officinalis
L.
were widely used in terms of their aromatic,
antiseptic antioxidant effects. Thus, the requirement of
rosemary cultivation and breeding was predicted to
increase day by day. Either the controversial
methods or modern biotechnologies, such as genetic
Figure 3 Effect of different bud’s length on rooting
Note: A: Roots induced from 2.5 cm length shoots; B: Roots
induced from 1.0 cm length shoots
Table 4 Propagation for adventitious shoots
Treatment
Medium formula
Observed results
No. of cultured shoots
No. of proliferating
shoots
Rate of proliferation
(%)
1
MS+6
-
BA 0.5 mg/L + NAA 0.5 mg/L
50
112
224
2
MS+6
-
BA 0.8 mg/L + NAA 0.5 mg/L
50
178
356
3
MS+6
-
BA 1.0 mg/L + NAA 0.5 mg/L
50
119
238
4
MS+6
-
BA 1.2 mg/L + NAA 0.5 mg/L
50
64
128
5
MS+6
-
BA 1.5 mg/L + NAA 0.5 mg/L
50
100
200
6
MS+6
-
BA 1.8 mg/L + NAA 0.5 mg/L
50
111
222
7
MS+6
-
BA 2.0 mg/L + NAA 0.5 mg/L
50
127
254
Table 5 Rooting induction for