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Bioscience Methods 2012, Vol.3, No.3, 21
-
26
http://bm.sophiapublisher.com
21
A Letter Open Access
Callus Induction and Plant Regeneration from Rosemary Leaves
Yumei Dong
1*
, Renxiang Wang
3*
, Zhengnan Li
1
, Cheng Qi
1
, Baogang Liu
1
, Rulan Duan
1
, Yating
Liu
1,2
1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, P.R. China;
2. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization, Huanggang Normal University, Huanggang,
438000, P.R. China
3. Science and technology training center of Kunming, Kunming, 650021, P.R. China
* These authors contributed equally to this work
Corresponding author email: liuyating999@yahoo.com.cn;
Author
Bioscience Methods 2012, Vol.3, No.3 doi: 10.5376/bm.2012.03.0003
Received: 05 Mar., 2012
Accepted: 03 May, 2012
Published: 08 May, 2012
This article was first published in Molecular Plant Breeding (Vol.10, No.2, 189-194) in Chinese, and here was authorized to translate and publish the paper in English
under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work
is properly cited.
Preferred citation for this article as:
Dong et al., 2012, Callus Induction and Plant Regeneration from Rosemary Leaves, Bioscience Methods, Vol.3, No.3 21
-
26 (doi: 10.5376/bm.2012.03.0003)
Abstract
Rosmarinus officinalis
L. leaves were used as explants in the study to investigate factors affecting callus induction and
plant regeneration. It was found that the culture medium with higher concentration of sucrose promoted callus induction. MS medium
supplemented with 6
-
BA 0.5 mg/L, NAA 0.5 mg/L, and sucrose 50 g/L showed the best result with an induction rate of 88.8%. When
regenerated callus develops shoots, it was found that MS medium containing 6
-
BA 1.5 mg/L, KT 0.5 mg/L and NAA 0.5 mg/L
showed relative good result with 50% regeneration rate. When grown in 6
-
BA 0.8 mg/L and NAA 0.5 mg/L, the amount of growth
was increased by more than 300%. MS medium containing NAA 0.1 mg/L showed better result in inducing roots with rooting rate
reaching 65%. Additionally, the study also showed that even though the explants were sterile, solution containing 75% ethanol with
other sterilizing reagents would result in mass explant deaths.
Keywords
Rosmarinus officinalis
L.; Leaf explant; Callus; Differentiation; Regeneration
Introduction
Rosmarinus officinalis
L. (Family Lamiaceae), which is
known as Rosemary, is a kind of perennial evergreen
shrub. It originates in Mediterranean region, and has
been cultivated as a common household plant around
the world for long time (Zhang et al
.
, 2006; Minaiyan
et al
.
, 2011).
R. officinalis
L. usually has three basic
function as raw materials. Firstly, it is used as spice
products in food and beverage, secondly, the
essential oil can be added into cosmetic production,
lastly, in terms of numerous antioxidants, the plant or
the essential oil is the materials of gastrointestinal
ailments, which has great effect in various spasmodic
conditions such as renal and biliary colic. So far,
numerous pharmacological studies have suggested
Rosmarinus officinalis
L. may have a high therapeutic
potential in inflammatory bowel diseases (Minaiyan
et al., 2011).
Nowadays, the nature extracts efficacy is a hot spot
research. For example, Rosa et al (2011) have
utilized ethanol extract from
R. officinalis
L to investigate
that the spasmolytic activity has relationship with
calcium channels switch in guinea pig ileum. Omri et
al (2011) have tested the expression of nucleoside
diphosphate kinase (NDPK) and heat shock protein
(HSP) was controlled by luteolin, carnosic acid, and
rosmarinic acid. Interestingly, essential oils from
R.
officinalis
L. are likely to kill mites (Martinez-Velazquez
et al., 2011), and antioxidant and antigenotoxic effects
of its extracts are observed in Salmonella typhimurium
TA98 and HepG2 cells (Zegura et al., 2011).
In food industrial, essential oils is coming from
natural plant, so, are often used as spices and
anticorrosive additives. The compounds of
R. officinalis
L. (such as Flavonoid, Phenolic, Piperitone, alpha-pinene,
Limoene and 1,8 -Cineole from ) are various due to
the plant species and the harvest time, especially in the
flowering stage (Papageorgiou et al
.
, 2008; Rasooli et
al., 2008). Gómez-Estaca et al (2010) have done an
antimicrobial research about that the effect of essential
oil from
R. officinalis
L. on 18 genera of bacteria, the
result showed that it had antibacterial function.
Rasooli et al (2008) have demonstrated that the natural
products of
R. officinalis
L. could inhibited the