Basic HTML Version
郭小勤等
, 2011,
稻瘟病菌对非寄主植物毛竹的接种试验
,
分子植物育种
Vol.9 No.8 (doi: 10.5376/mpb.cn.2010.09.0008)
1060
种后均置于暗室
(25
℃
)
保湿
24 h
，保持相对湿度近
100%
，然后重新放置培养室中以前述条件生长。
5
天后观察症状。在进行破坏蜡质层实验时，用牙签
将蜡质层刺破，后续接种与上同。
3.3
组织染色镜检
为观察真菌的发育情况，对样本进行锥虫蓝
(trypan blue)
染色。将毛竹和水稻叶片剪成
1.5 cm
左
右小块，置于
Farmer
氏溶液
(
乙酸
:
乙醇
:
氯仿＝
1: 6:
3, v/v/v)
中透明化，然后在含
0.05%
锥虫蓝的乳酚与
乙醇
(1: 2, v/v)
混合液中染色，再用水漂洗并浸于饱
和水合氯醛中脱色。叶片放置于滴有
50%
甘油的载
玻片上，用
Zeiss Axioskop2 plus
显微系统镜检，并
用
Zeiss Axiolam HRC
相机组件拍摄图片。
作者贡献
郭小勤是本研究的实验设计和实验研究的执行人；汤
定钦和方伟参与实验设计，试验结果分析；桂仁意是项目的
负责人，指导实验设计，数据分析，论文写作与修改。全体
作者都阅读并同意最终的文本。
致谢
本研究由浙江省自然科学基金
(2451001121)
，浙江省重
大科技专项
(2008C12067
-
1)
和国家
863
项目
(2007AA021403)
共同资助资助。感谢两位匿名的同行评审人的评审建议和修
改建议。
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