Basic HTML Version
谢树章等
, 2011,
内含子调控转基因植物外源基因的表达研究
,
分子植物育种
Vol.9 No.12 (doi: 10.5376/mpb.cn.2011.09.0012)
1091
续表
2
Continuing table 2
商业化转化体
Commercial transformants
目的基因
Aim gene
启动子
Promote
内含子与其他调控元件
Intron and other regulatory elements
大豆
MON89788
Soybean MON89788
P-FMV/TSF1
拟南芥
tsf1
基因前导内含子，拟南芥叶绿体转
运肽序列
A. thaliana tsf1 leader and intron; chloroplast
transit peptide fromA. thaliana
大豆
GTS40
-
3
-
2
Soybean GTS40
-
3
-
2
EPSPS
CaMV35S
叶绿体转运肽
4
CTP4
棉花
15985
Cotton 15985
1. cry1Ac
2. cry2Ab
1.double CaMV35S
2. double CaMV35S
2.
热休克蛋白
70
内含子，叶绿体转运肽
2
2. HSP70 leader sequence, CTP2
棉花
COT102
Cotton COT102
vip3A(a)
改造启动子
Modified promoter
拟南芥肌动蛋白
2
基因第一外显子和内含子
First exon and intron of At actin
-
2 gene
棉花
GHB614
Cotton GHB614
拟南芥启动子结构域组蛋白
H4
基因
Promoter region of the histone
H4 gene fromA. thaliana
拟南芥
h3At
内含子
h3At intron
作者贡献
谢树章参与题目选定、文献资料收集和梳理分析、初
稿撰写与修改润色；李新海、雷开荣参与总体思路和文章结
构的确立、文章结构修改、文字润色。全体作者都阅读并同
意最终的文本。
致谢
本研究由国家转基因生物新品种培育重大专项课题
(2008ZX08011
-
003)
和重庆市科技攻关计划项目
(CSTC,
2009AB1110)
共同资助。
参考文献
Alejandro F., Pedro L.R., Elvira F., Danay C., Yadira S., Rudy P.,
Raidel R and Merardo P., 2006, Intron-hairpin RNA derived
from replication associated protein C1 gene confers
immunity to tomato yellow leaf curl virus infection in
transgenic tomato plants, Transgenic Research, 15:291-304
An Y.Q.C., and Meagher R.B., 2010, Strong expression and
conserved regulation of ACT2 in Arabidopsis thaliana and
physcomitrella patens, Plant Mol. Biol. Rep., 28:481-490
Bartlett J.G., Snape J.W., and Harwood W.A., 2009,
Intron-mediated enhancement as a method for increasing
transgene expression levels in barley, Plant Biotechnology
Journal, 7 (9): 856-866
Bhattacharyya S., Pattanaik S., and Maiti I.B., 2003, Intron-mediated
enhancement of gene expression in transgenic plants using
chimeric constructs composed of the Peanut chlorotic
streak virus (PClSV) promoter–leader and the antisense
orientation of PClSV ORF VII (p7R), Planta, 218: 115-124
Borokov A.Y., 1997, Role of the leader intron in regulation of
the expression of the potato sucrose synthase gene, Plant
Physiol, 114(Sl): 64-71
Bourdon V., Harvey A., and Lonsdale D.M., 2001, Introns and
their positions affect the translational activity of mRNA
inplant cells, Scientific Report, 2(5): 394-39
Brinster R.L., Alien J.M., Behringer R.R., Gelinas R.E., and
Palmiter R.D, 1988, Introns increase transcriptional
efficiency in transgenic mice, Proc. Natl. Acad. Sci., USA.,
85(3): 836-840
Cabello J.V., Dezar C.A., Manavella P.A., and Chan R.L., 2007,
The intron of the Arabidopsis thaliana COX5c gene is able to
improve the drought tolerance conferred by the sunXower
Hahb-4 transcription factor, Planta, 226: 1143-1154
Callis J., Fromm M., and Walbot V., 1987, Introns increase
gene expression in cultured maize cells, Genes and
Development, l: 1183-1200
Chaubet G.N., Kapros T., Flenet M., Kahn K., Gigot C., and
Waterborg J.H., 2001, Tissue-dependent enhancement of
transgene expression by introns ofreplacement histone H3
genes of Arabidopsis, Plant Molecular Biology, 45: 17-30
Clancy M., and Hannah L.C., 2002, Splicing of the maize Sh1
first intron is essential for enhancement of gene expression,
and a T-rich motif increases expression without affecting
splicing, Plant Physiol, 130(2): 918-929
Ding H.M., Shao G.B., and Xu Y.X., 2006, The function of