Journal of Mosquito Research 2015, Vol.5, No.18, 1-8
4
Table 1 Per cent larvicidal activity of
Murraya koenigii
hexane leaf extract isolated fractions
against vector mosquitoes
Concentration (ppm)
Fractions
A
B
C
D
E
F
Aedes aegypti
25
20.8±3.34(27.1)
b
09.6±2.19(18.1)
a
06.4±2.19(14.7)
a
44.8±3.34(42.0)
c
25.6±2.19(30.4)
b
24.8±1.78(29.9)
b
50
30.4±2.19(33.5)
c
18.4±2.19(25.4)
b
12.0±2.82(20.3)
a
59.2±3.34(50.31)
e
40.8±1.78(39.7)
d
38.4±2.19(38.3)
d
75
43.2±1.78(41.1)
b
20.0±2.82(26.6)
a
17.6±2.19(24.8)
a
84.4±4.0(66.7)
d
51.2±3.34(45.7)
c
50.4±3.57(45.2)
c
100
50.4±3.57(45.2)
c
31.2±3.34(33.9)
b
19.2±1.78(25.9)
a
100.0±00(90.0)
f
75.2±1.78(60.1)
e
64.8±1.79(53.6)
d
Control
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
Anopheles stephensi
25
19.2±4.38(25.9)
b
13.6±2.19(21.6)
a
11.2±1.78(19.6)
a
38.4±2.19(38.3)
d
28.8±1.78(32.5)
c
21.6±3.57(27.7)
b
50
27.2±1.78(31.4)
c
20.0±2.82(26.6)
b
13.6±2.19(21.6)
a
48.8±3.34(44.3)
e
36.8±3.34(37.4)
d
36.8±3.34(37.4)
d
75
39.2±5.21(38.8)
b
21.6±3.57(27.7)
a
15.2±1.78(22.9)
a
79.2±5.21(62.9)
d
55.2±3.34(47.9)
c
40.8±3.34(39.7)
b
100
48.8±5.93(44.3)
c
28.8±1.78(32.5)
b
19.2±3.34(25.9)
a
97.6±2.19(81.1)
e
68.0±2.82(55.6)
d
46.4±4.56(42.9)
c
Control
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
Culex quinquefasciatus
25
30.4±4.56(33.5)
c
16.8±3.34(24.2)
a
14.4±2.19(22.3)
a
52.8±3.34(46.6)
d
26.4±4.56(30.9)
bc
21.6±3.57(27.7)
ab
50
39.2±5.21(38.8)
b
21.6±3.57(27.7)
a
19.2±3.34(25.9)
a
65.6±4.56(54.1)
d
37.6±3.57(37.8)
b
46.4±4.56(42.9)
c
75
54.4±3.57(47.5)
c
31.2±4.38(33.9)
b
21.6±2.19(27.7)
a
88.8±3.34(70.5)
d
50.4±2.19(45.2)
c
52.0±2.82(46.1)
c
100
60.8±1.78(51.2)
c
41.6±3.57(40.2)
b
25.6±2.19(30.4)
a
99.2±1.78(84.9)
d
56.8±3.38(48.9)
c
60.8±3.34(51.2)
c
Control
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
0.0±0.0(0.0)
a
Values are mean (%) of five-replicates of three trials ±standard deviation. Figures in parentheses are angular transformed. Different superscript alphabets indicate statistical significant difference
at P <0.05 levels by Two way ANOVA followed by Tukey’s test
Table 2 Probit analysis of larvicidal activity of
Murraya koenigii
hexane leaf extract isolated fractions against vector mosquitoes
Vector
mosquitoes
Aedes aegypti
Anopheles stephensi
Culex quinquefasciatus
Fractions
LC
50
(ppm)
95% CL
LC
90
(ppm)
95% CL
LC
50
(ppm)
95% CL
LC
90
(ppm)
95% CL
LC
50
(ppm)
95% CL
LC
90
(ppm)
95% CL
LL
UL
LL
UL
LL
UL
LL
UL
LL
UL
LL
UL
A
95.65
88.86
104.65 210.23 186.97 243.90 101.51 94.16
111.39 213.81 190.24 247.84
71.61
66.48
77.33
188.20 168.20 216.99
B
151.68 134.55 178.78 281.38 240.06 348.06 185.79 154.31 248.44 378.39 298.82 539.13
122.16 111.06 138.44 246.88 215.03 295.45
C
196.69 165.60 254.55 350.37 283.66 475.85 298.73 215.47 579.35 586.19 402.02 1210.78 225.54 175.67 351.29 473.32 348.70 791.00
D
35.06
29.47
39.65
80.43
74.61
88.09
42.51
37.35
46.96
89.52
83.01
98.18
27.20
20.70
32.30
76.07
70.62
83.12
E
65.38
62.01
68.83
140.82 131.64 152.44 67.79
63.82
71.97
157.77 145.23 174.35
79.97
74.44
86.68
197.66 176.04 228.97
F
73.00
68.86
77.56
165.02 151.38 183.21 104.40 94.55
119.16 254.32 217.02 314.94
72.54
66.58
79.38
171.45 151.53 201.97
