International Journal of Horticulture 2015, Vol.5, No.12, 1
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Table 5 List of the primers and the degree of the polymorphism obtained among the 14 squash genotypes
Primer
Sequence 5'-3'
Total of bands (a)
Polymorphic bands (b)
Polymorphic % (b/a x 100)
A01
CAGGCCCTTC
45
45
100
A02
TGCCGAGCTG
7
7
100
A03
AGTCAGCCAC
18
18
100
A04
AATCGGGCTG
29
29
100
A05
AGGGGTCTTG
27
27
100
A06
GGTCCCTGAC
8
8
100
A07
GAAACGGGTG
26
26
100
A08
GTGACGTAGG
20
20
100
A09
GGGTAACGCC
26
26
100
A10
GTGATCGCAG
3
3
100
Mean
20.9
20.9
100
Table 6 Similarity coefficient matrix among the 14 squash genotypes based on 10 RAPDs markers
G1
G2
G3 G4
G5
G6
G7
G8
G9
G10
G11
G12
G13
G2
.880
G3
.824
.869
G4
.753
.813
.854
G5
.843
.850
.891
.828
G6
.801
.824
.835
.816
.854
G7
.719
.749
.745
.674
.772
.738
G8
.757
.764
.753
.712
.772
.790
.768
G9
.772
.787
.813
.742
.801
.820
.708
.723
G10
.783
.835
.846
.775
.828
.809
.742
.734
.787
G11
.794
.824
.828
.772
.831
.813
.760
.730
.775
.809
G12
.787
.801
.820
.749
.824
.805
.738
.723
.775
.787
.858
G13
.820
.843
.839
.790
.850
.846
.749
.779
.787
.820
.831
.839
G14
.794
.809
.820
.787
.824
.828
.715
.730
.753
.794
.805
.798
.891
G1: PI 506466; G2: PI 292014; G3: PI 518688; G4: PI 506467; G5: PI 615119; G6: PI 136448; G7: PI 518687; G8: PI 216032; G9:
Matrouhy; G10: Butternut; G11: Yellow Crookneck; G12: Shamamy; G13: Copi; G14: Eskandrani
genotypes was wide with percentage of variation as
520%, there was no clear trend among tested squash
morphotypes. This may be explained because of the
overall low level of ascorbic acid content in squash
compared with other vegetables such as pepper, broccoli,
strawberry and spinach (Lee and Kader 2000).
Our results suggest that RAPD markers are good
alternative for the evaluation of genetic diversity and
assessing the separation among landraces belonging to
different geographical regions. The level of polymorphism
among the
Cucurbita pepo
L. genotypes was extremely
high. This high level of RAPD markers polymorphism in
Cucurbita pepo
genotypes is in accordance with the
results of Hadia et al. (2008) and Tsivelikas et al. (2009),
who reported that
Cucurbita pepo
is a highly polymorphic
species. In an earlier study, Hadia et al. (2008) reported
89% polymorphism among
Cucurbita pepo
genotypes.
Lower percentage of polymorphism (61%) has been
detected in
Cucurbita pepo
by El-Adl et al., (2012) which
can be due to the low number of primers (6 primers)
and genotypes (7 genotypes) used in such study. Also,
a low level of polymorphism in
Cucurbita pepo
was
described by Al-Tamimi, (2014) and most likely for
using low number of primers and genotypes.
The variation assessed by 209 polymorphic RAPD
bands generated by 10 primers that screened the 14
genotypes. The DNA fragments generated by RAPD
primers were different in number, intensity and
position indicating high genetic variation among the
studied genotypes. Primer A01 provided the highest
