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International Journal of Molecular Veterinary Research
35
In June 2006, we sampled two stomach contents and
five fetal membranes, two of which have a lot of white
rice-like granules in them, from aborted fetuses on the
pig farm in Longyan City, for detecting
Chlamydia
sp.
by means of nested polymerase chain reaction
(nested-PCR) developed by us.
The two sets of primers for
C. psittaci
MOMP gene
used in nested-PCR are:
The primer pair No.1:
MP1: 5’-ATGAAAAAACTCTTGAAATCGGCA-3’
MP2: 5’-TTAGAATCTGAATTGAGCATTCATGTG-3’
The primer pair No.2:
MP3: 5’-CAGGATACTACGGAGATTATGTTT-3’
MP4: 5’-GATTAGATTGAGCGTATTGGAA-3’
The result of the seven aborted pig samples examined
by nested-PCR are seen Figure 2.
Figure 2 The result of the seven aborted pig samples examined
by nested-PCR for
Chlamydia
sp. DNA
Note: M: DL2000 Marker; 1: the positive control; 2~3: aborted
pig fetus’s fetal membranes with a lot of white rice-like
granules; 3~4: aborted pig fetus’ stomach contents; 6~8:
aborted pig fetus’s fetal membranes without any white rice-like
granulesa
From the Figure 2, we know that the MOMP gene
fragments which correspond with the expected that in
size (488 bp), were obtained from 1/2 aborted pig
fetus’s fetal membranes with a lot of white rice-like
granules (the sample No.3), 2/2 aborted pig fetus’
stomach contents (the samples No.4 and No.5), and
2/3 aborted pig fetus’s fetal membranes without any
white rice-like granules (the samples No.6 and No.7).
2 Discussion
On the basis of the results of the serological test and
PCR, it was confirmed that chlamydiosis existed in
swine herds on the pig farm in in Longyan City,
Fujian province. But while pregnant sows were
infected by chlamydia, white rice-like granules
pathologic change may appear or may not appear in
aborted pig fetus’s fetal membranes. Formation of
white rice-like granules in placenta may be related
with chlamydia infection time. How do the pathologic
changes occur will be studied further.
I
n some literature on pathological changes of fetal
membrane of pig chlamydial abortion, it was
described that fetal membranes of pig chlamydial
abortion showed black-red colour with water-like
material covering on them and there necrotic focus in
the mucous membrane face of placenta and edema
around the necrotic focus (Pienaar et al., 1975; Yang,
1982 and 1984), but no recording same as that
mentioned above.
Acknowledgement
The test was subsidized by The national Fun (2008FY210200).
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