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Leukemic T-cell Precursors from T-lineage All Patients Characterized by Profound Ku80 Deficiency
5
Figure 2 Effects of
Ku
Expression Levels on Expression Levels of Lymphoid-Priming Genes in Primary Lymphocyte Precursors
from ALL Patients
Note: A one-way agglomerative hierarchical clustering technique was used to organize expression patterns using the average distance
linkage method such that IK-regulated lymphoid priming genes (18) (rows) having similar expression across patients were grouped
together (average distance metric). The heat map depicts expression values represented by standard deviation units above (red) and
below the mean (green). Dendrograms were drawn to illustrate similar gene-expression profiles from joining pairs of closely related
gene expression profiles, whereby genes joined by short branch lengths showed most similarity in expression profile across patients.
[B] Co-regulation of
IKZF1
,
Ku
and Lymphoid-priming genes in Primary Lymphocyte Precursors from ALL patients. Expression
values expressed as Standard Deviation units calculated from 1104 samples were compiled for the 5 studies and rank ordered
according to the mean expression of three highly correlated transcripts (208642_s_at (
XRCC5
), 208643_s_at (
XRCC5
), 200792_at
(
XRCC6
). These samples were also rank ordered according to
IKZF1
expression level (205038_at, 205039_s_at, 216901_s_at,
227344_at and 227346_at; 3 of these were common in all Affymetrix platforms (205038_at, 205039_s_at, 216901_s_at)). T-tests
were performed for the combined Standard Deviation units from the 5 datasets (2-sample, Unequal variance correction,
p-values<0.05 deemed significant) to reveal 13 transcripts significantly upregulated in specimens with both high
Ku
and high
IKZF1
expression
Molecular Medical Science, Int’l Journal of