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Genomics and Applied Biology
2013, Vol.4, No.4, 22-34
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Relationship Between SSR-Based Molecular Marker and Cotton F
1
Inter
Specific Hybrids Performance for Seed Cotton Yield and Fiber Properties
Yanal A. Alkuddsi , S.S. Patil , S.M. Manjula , H.L Nadaf , B.C. Patil
Agricultural Research Station, University of Agricultural Sciences, Dharwad- 580005, Karnataka, India
Corresponding Author email:
y.alkuddsi@hotmail.com;
Author
Genomics Appied Biology, 2013, Vol.4, No.4 doi: 10.5376/gab.2013.04.0004
Copyright
© 2013 Alkuddsi et al. This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted
use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Knowledge of genetic diversity and relationships among breeding materials has a significant impact on crop improvement.
Association between parental divergence and progeny performance has not been well documented in cotton (
Gossypium hirsutum
L.).
The objectives of this study were to estimate genetic diversity among selected cotton genotypes on the basis of simple sequence
repeat (SSR) markers, and to investigate the relationship between genetic diversity and F
1
population performance and heterosis.The
present study was conducted to investigate the relationship between parents molecular marker diversity and interspecific hybrids of
cotton to evaluate the hybrid performance and heterosis using molecular markers. Twenty eight F
4
lines of (
Gossypium barbadense
L.)
were crossed with the four common diverse testers (
Gossypium hirsutum
L.) viz., DH 98-27 (T
1
), ZCH8 (T
2
), 178-24 (T
3
) and DH
18-31 (T
4
) to produce 112 F
1
inter specific hybrids during 2010. These 112 F
1
hybrids, their F
5
barbadense lines with 4 hirsutum
testers and ruling commercial check (MRC6918 and DCH32) were evaluated for yield and fiber quality traits and sown during kharif
2011 at University of Agricultural Sciences, Dharwad, India. Genetic distances (GD) among the parents were calculated from 40
microsatellite marker data, and their correlation with hybrid performance and heterosis were analysed. The dendrogram constructed
from the pooled data revealed three distinct clusters. One cluster involved testers and other clusters showed all lines were placed
which are already having proven record in giving good hybrids. The similarity coefficient values between the line DB 533
×
DB 534
F
4
IPS 49 and the tester DH 98-27 showed 67%. It revealed that DB 533
×
DB 534 F
4
IPS 49 was closely related to DH 98-27 with
67% similarity between parents. The hybrid between DB 533 x DB 534 F
4
IPS 49 and DH 98-27 exhibited the highest yield of
2884.26 kg/ha. Similarity coefficient (88%) value between lines and testers showed between the line DB 533
×
DB 534 F
4
IPS 52
and the tester ZCH8, the hybrid between these recorded an yield of 2 040.757 kg/ha. Lowest similarity coefficient value was noticed
between the line DB 533
×
DB 534 F
4
IPS 16 and tester DH 98-27 which revealed that they are far distinct from each other. This
combination exhibited 2384.62 kg/ha yield. Genetic distance (GD) ranged from 0.041 to 0.429, with an average of 0.183. The result
implied that each cluster dendrogram substantially reflected its own genetic relationship among parents. Overall, a low significant
correlation of GD with hybrid performance and heterosis was detected in Table 2. Highly significant positive correlation were found
between genetic distance (GD) and ginning outturn for F
1
performance (0.277) and heterosis over MRC 6918 (0.279) and DCH 32
(0.279), while significant positive correlation were found between genetic distance (GD) and ginning outturn for mid parent heterosis
(0.237). Highly significant positive correlation were found between genetic distance (GD) and seed cotton yield for F
1
performance
(0.359) and heterosis over
Bt
check MRC 6918 (0.336) and over non
Bt
check DCH 32 (0.362), while significant positive correlation
were found between genetic distance (GD) and seed cotton yield for mid parent heterosis (0.226). Significant positive correlation
were found between genetic distance (GD) and lint index for mid parent heterosis (0.227), F
1
performance (0.251) and heterosis over
MRC 6918 (0.250) and DCH 32 (0.250), while significant positive correlation were found only between genetic distance (GD) and
fiber micronaire value for F
1
performance (0.241).
Keywords
Gossypium hirsutum
;
Gossypium barbadense
; SSR marker; Genetic distance (GD)
Preferred citation for this article:
Alkuddsi et al., 2013, Relationship Between SSR-Based Molecular Marker and Cotton F
1
Inter Specific Hybrids Performance for Seed Cotton Yield and
Fiber Properties, Genomics and Applied Biology, Vol.4, No.4 22-34 (doi: 10.5376/gab.2013.04.0004)
Received: 29 Jun., 2013
|
Accepted: 16 Jul., 2013
|
Published: 29 Oct., 2013
Genomics and Applied Biology