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Genomics and Applied Biology
29
2.4 Analysis of SSR profiles
Pair similarity coefficients were calculated for all
pairwise combinations of the parental lines according
to the method developed by Nei and Li (1979):
Sij=
2Nij / (Ni+ Nj)
, where
Sij
is the similarity between
parents
i
and
j
;
Nij
is the number of bands present in
both parents;
Ni
is the number of bands present only
in parent I;
Nj
is the number of bands present only in
parent
j
. GD (genetic distance) was calculated as GD=
1-
Sij
. The similarity matrix from SSR markers, which
were computed using NTSYS-PC version 2.1 (Rohlf,
2001) were used to construct dendrograms based on
UPGMA (the unweighted pair- group method with
arithmetic means). Using the same NTSYS software, a
cophenetic value matrix was calculated to test the
goodness of fit for the cluster analysis to the original
distance matrix.
For studying the relationship between SSR molecular
maker and hybrids performance and heterosis, the mid
parent heterosis (MPH) was computed using the
formula 100 × (F
1
-MP)/MP, where F
1
is the hybrid
performance and MP is the mid-parent mean. Per cent
heterosis in F
1
over commercial check (CC) was
computed using the formula 100 × (F
1
-CC)/CC, where
CC commercial check mean.
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