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Cotton Genomics and Genetics
50
8 X (DB 533 x DB 534 F
4
IPS 30) (-33.29) exhibited
lowest negative heterosis over MRC 6918. Twenty
one crosses showed significant heterosis in positive
direction over MRC 6918. In case of DCH 32 non
Bt
check, the cross DH 98-27 X (DB 533 x DB 534 F
4
IPS 32) (45.34) showed highest significant positive
heterosis over this check, but the cross ZCH 8 X (DB
533 x DB 534 F
4
IPS 30) (-46.91) recorded lowest
significant negative heterosis value over DCH 32
commercial check. Seventeen crosses exhibited
significant negative heterosis over DCH 32 commercial
check and only one cross showed significant positive
heterosis.
2 Materials and Method
To create recombinational variability for combining
ability, the elite barbadense lines DB 533 and DB 534
were crossed during 2007~2008. During two seasons
2008~2009 and 2009~2010 these barbadense crosses
were advanced to F
2
and F
3
generations, respectively.
The F
3
lines were evaluated for productivity and fiber
quality parameters realizing the emphasis laid on
developing ELS (Extra Long Stable) cotton hybrids
out of 171 F
3
lines, only those F
3
lines with acceptable
fiber strength were utilized in the study on
recombinational variability of combining ability.
During 2010~2011 those twenty eight F
4
lines of
barbadense cross DB 533 × DB 534 depending on the
higher value of fiber tenacity, were crossed with the
selected four hirsutum testers viz., DH 98-27 (T
1
),
ZCH 8 (T
2
), 178-24 (T
3
) and DH 18-31 (T
4
) selected
based on earlier study. Each barbadense F
4
line was
involved in a set of crosses (112 crosses refer to as
derived F
1
crosses) were subjected to Line x Tester
analysis.
Heterosis of F
1
over mid parent (MP) and commercial
check (MRC 6918 and DCH 32) were calculated by
methods of Turner (1953) and Hayes et al. (1955) as
given below.
Per cent heterosis in F
1
over mid parent (MP) = (F
1
-
MP)/MP × 100
Where, Mid parent (MP) = (P
1
+ P
2
)/2
Per cent heterosis in F
1
over commercial check (CC) =
(F
1
- CC)/CC × 100
Where, MP = Mid parent, CC = Commercial check
Mean sum of squares due to error from RBD analysis
was considered to compute standard error (S.E.) of
estimated heterosis as follows.
S.E. for heterosis over mid parent
S.E. (Hmp) = [(3/2 × EMS)/r]
0.5
S.E. for heterosis over commercial check
S.E. (Hcc) = (2 EMS/r)
0.5
Where, EMS = Error mean sum of squares
The critical difference values in each case were
worked out by multiplying their corresponding S.E.
values with table ‘t’ value at error degree of freedom
at 5 and 1 per cent levels of significance.
References
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1
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5
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(
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http://dx.doi.org/10.1016/S1161-0301(99)00018-0
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th
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Cotton Production – A Global Vision 1
Cotton Genomics and Genetics