Bt Research (Online) 2010, Vol.1 No.2
http://bt.sophiapublisher.com
- 10 -
different plasmid profiles, cry genotypes and crystal
proteins. Thus, it is believed that
Bt
W015
-
1 could
be used as a potential biopesticide alternative to
Btk
.
Authors’ contributions
LX, WFZ and ZML conducted all the research for this paper. YGC was
involved in collecting the diseased silkworm larvae. LX and WFZ jointly
completed the data analysis and manuscript preparatin. YZL participated
in experimental management and reviewed the manuscript. XJF
coordinated the project and was fully involved in the experimental
design, data analysis and manuscript preparation. All authors read and
approved the final manuscript.
Acknowledgements
Authors would like to thank Madam Ruying Zhao from the Jiaxing
Bureau of Agriculture in Zhejiang Province for her help in collecting the
diseased silkworm larvae. We greatly appreciate Dr. Phil Grau, Sr.
Entomologist of SynTech Research, for reading and revising the
manuscript. We also thank two anonymous reviewers for their strict
criticism on this paper. This work was initiated by the China National Bt
Collection Initiative project and partly supported by the National 863
Program of China (Project No. 2004AA2111112). Mention of trade
names or commercial products in this paper is solely for the purpose of
providing specific information and does not imply recommendation or
endorsement by authors or institutes or University involved in this study.
References
Aronson J.N., and Thompson F.M., 1971,
Bacillus thuringiensis
sporulation at suboptimal temperature, J . Bacteriol., 105: 445-448
Carlson C.R., Johansen T., and Kolsto A.B., 1996, The chromosome map
of
Bacillus thuringiensis
subsp.
canadensis
HD224 is highly similar
to that of the
Bacillus cereus
type strain ATCC 14579, FEMS
Microbiol . Lett., 141: 163-167
Crickmore N., Zeigler D.R., Feitelson J., Schnepf E., Van Rie J.,
Lereclus D., Baum J., and Dean D.H., 1998, Revision of the
nomenclature for the
Bacillus thuringiensis
pesticidal crystal
proteins, Microbiol. Mol. Biol. Rev., 62: 807-813
Khajuria C., Zhu Y.C., Chen M.S., Buschman L.L., Higgins R.A., Yao
J.X., Crespo A.L.B., Siegfried B.D., Muthukrishnan S., and Zhu
K.Y., 2009, Expressed sequence tags from larval gut of the
European corn borer (
Ostrinia nubilalis
): Exploring candidate genes
potentially involved in
Bacillus thuringiensis
toxicity and resistance,
BMC Genomics, 10: 286
Knowles B.H., 1994, Mechanism of action of
Bacillus thuringiensis
insecticidal d-endotoxins,Advances in Insect Physiology, 24: 275-308
Kuo W.S., and Chak K.F., 1996, Identification of novel cry type genes
from
Bacillus thuringiensis
strains on the basis of restriction
fragment length polymorphism of the PCR amplified DNA, Applied
and Environmental Microbiology, 62: 1369-1377
Kristensen N.P., Scoble M.J., and Karsholt O., 2007, Lepidoptera
phylogeny and systematics: The state of inventorying moth andbutterfly
diversity, Zootaxa, 1668: 699-747
Laemmli U.K., 1970, Cleavage of structural proteins during the
assembly of the head of bacteriophage T4, Nature, 227: 680-685
Lecadet M.M., Blondel M.O., and Ribier J., 1980, Generalized
transduction in
Bacillus thuringiensis
var.
berliner
1715 using
bacteriophage CP
-
54Ber, J. Gen. Microbiol., 121: 203-212
Lowry O.H., Rosebrough N.J., Farr A.L., and Randall R.J., 1951, Protein
measurement with the folin phenol reagent, J. Biol. Chem., 193: 265-275
Mita1 K., Kasahara M., Sasaki S., Nagayasu Y., Yamada T., Kanamori H.,
Namiki N., Kitagawa M., Yamashita H., Yasukochi Y., Kadono-
Okuda K., Yamamoto K., Ajimura M., Ravikumar G., Shimomura
M., Nagamura Y., Shin-i T., Abe H., Shimada T., Morishita S., and
Sasaki T., 2004, The genome sequence of silkworm,
Bombyx mori
,
DNA Res., 11(1): 27-35
Ohba M., 1996,
Bacillus thuringiensis
populations naturally occurring on
mulberry leaves: A possible source of the populations associated
with silkworm-rearing insectaries, Journal of Applied Microbiology,
80(1): 56-64
Porcar M., Iriarte J., Cosmao Dumanoir V., Ferrandis M.D., Lecadet M.,
Ferre J., Caballero P., 1999, Identification and characterization of
the new
Bacillus thuringiensis
serovars pirenaica (serotype H57)
and iberica (serotype H59), J. Appl. Microbiol., 87: 640-648
Sayyed A.H., Crickmore N., and Wright D.J., 2001, Cyt1Aa from
Bacillus thuringiensis
subsp.
israelensis
is toxic to the diamondback
moth, Plutella xylostella, and synergizes the activity of Cry1Ac
towards a resistant strain, Appl. Environ. Microbiol., 67: 5859-5861
Triglia T., Peterson M.G., and Kemp D.J., 1988, A procedure for
in vitro
amplification of DNA segments that lie outside the boundaries of
known sequences, Nucleic Acids Res., 16: 8186
Vilas-Boas G.T., Lemos M.V., 2004, Diversity of cry genes and genetic
characterization of
Bacillus thuringiensis
isolated from Brazil, Can.
J. Microbiol., 50: 605-613
Xie L., Zhang W.F., Quan J.X., Liu Z.M., Ye D.W., Li Y.Z., and Fang
X.J., 2009,
Bacillus thuringiensis
collection and isolates identifyca-
tion from Damingshan and Dawangling natural reserves in Guangxi
province, Jiyinzuxue Yu Yingyong Shengwuxue (Genomics and
Applied Biology), 28(1): 62-68
Yu H., Zhang J., Huang D., Gao J., and Song F., 2006, Characterization
of
Bacillus thuringiensis
strain Bt185 toxic to the Asian cockchafer:
Holotrichia parallela, Plasmid, 53: 13-17
Zhang W.F., Quan J.X., Xie L., Wang X., Yi Y.T., Feng M.M., Zhu L.,
Wang R.P., and Fang X.J., 2009, Collection of
Bacillu
s and
identification of
Bacillus thuringiensis
isolates from tropical rain
forest reserves of Hainan island, Jiyinzuxue Yu Yingyong Shengwu-
xue (Genomics and Applied Biology), 28(2): 265-274