Molecular Pathogens 2012, Vol.3, No.3, 12
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p://mp.sophiapublisher.com
15
Figure 6 Homology comparison of 42 cloned squences with
NC_001340 (Puchta et al., 1990)
heavily in Yanqi County.
1.5
The ASSVd distribution of leaf tissues in four
kind fruit trees
Blue-purple positive signals were detected in the
nucleus of palisade tissues while it was not detected in
control experiments (Figure 7). This data further showed
that ASSVd was in the nulceus of leaf tissue cells.
2
Discussion
ASSVd can be spread via grafting and prune tools, it
Table 2 Geographical distribution of viroids detected in Xinjiang
province, China for plants assayed by RT-PCR for ASSVd
Region
No. of the tested
samples
No. of the tested
positive samples
Yanqi
410
35
Hejing
90
0
Bohu
140
0
Korla
410
9
Heshuo
320
6
Xinhe
80
0
Aksu
260
1
Total
1710
51
would cause a loss to fruit tree production if it widely
spread. Although there is no effective method to
prevent occurrence of the disease, we could discover
the source of infection and handle it in time though
suitable molecular detection methods.
In our study, the RT-PCR results showed that apple
scar skin disease have occurred in Xinjiang of China.
It has been shown that ASSVd can infect apple and
pear tree (Zhu et al., 1995; Puchta et al., 1990; Guo et
al., 2005). But there was no report that ASSVd was