JOURNAL OF MOSQUITO RESEARCH
93
Table 3 Dose response larvicidal assay using acetone extract of
A. aspera
foliage against Cx.
vishnui
group
Mortality rate ( Mean ± SE)
Larval instars
Concentrations (ppm)
24 h
48 h
72 h
20
33.32 ± 0.47
40.00±0.82
46.64± 0.47
40
42.64 ± 0.94
49.32± 1.7
64.00± 0.82
60
72.00 ± 1.41
84.00± 0.82
90.64± 0.47
80
82.64 ± 0.94
98.64 ± 0.47
100.00±0.00
First
100
89.32 ± 0.47
100.00 ±0.00
100.00 ± 0.00
20
30.64 ± 0.47
33.32 ± 0.47
46.64 ± 1.24
40
37.32 ± 0.47
38.64 ± 0.47
61.32 ± 1.24
60
65.32 ± 1.7
69.32 ± 0.94
81.32 ± 1.24
80
78.64 ± 0.47
89.32 ± 0.47
100.00 ± 0.00
Second
100
81.32 ± 0.47
100.00 ± 0.00
100.00 ± 0.00
20
25.32 ± 0.47
29.32 ± 0.47
36.00 ± 0.82
40
34.64 ± 0.94
40.00 ± 0.82
49.32 ± 0.94
60
60.00 ± 1.41
62.64 ± 1.25
72.00 ± 0.82
80
70.64 ± 0.47
78.64 ± 0.94
84.00 ± 0.82
Third
100
78.64 ± 0.47
93.32 ± 0.47
97.32 ± 0.94
20
14.64 ± 0.47
17.32 ± 0.47
21.32 ± 0.47
40
26.64 ± 0.47
30.64 ± 0.94
34.64 ± 0.47
60
54.64 ± 1.25
56.00 ± 1.41
61.32 ± 1.25
80
60.00 ± 0.82
64.00 ± 0.82
66.64 ± 0.47
Fourth
100
66.64 ± 0.47
72.00 ± 0.82
74.64 ± 0.47
Table 4 Assessment of LC
50
and LC
90
values of acetone extract of
A
.
aspera
through log-probit and regression analyses
Larval instars
Period of exposure LC
50
LC
90
Regression
R
2
- value
24
35.46
111.41
0.19x + 4.6
0.94
48
28.60
66.52
0.21x + 5.9
0.91
1
st
72
24.44
55.92
0.18 x + 9.37
0.89
24
40.72
147.89
0.18x + 3.97
0.93
48
35.10
87.18
0.23 x + 2.73
0.96
2
nd
72
24.69
67.48
0.18 x + 8.57
0.94
24
47.30
176.51
0.18x + 2.77
0.96
48
39.28
115.14
0.21x + 2.7
0.99
3
rd
72
32.15
93.72
0.20x + 5.13
0.99
24
63.39
213.34
0.17x + 0.83
0.92
48
56.74
198.73
0.18x + 1.3
0.95
4
th
72
50.35
187.06
0.17x + 2.53
0.94
Note: x = concentration (in ppm) of solvent extracts
Journal of Mosquito Research