Basic HTML Version
JOURNAL OF MOSQUITO RESEARCH
91
Table 1 Dose response larvicidal assay using crude extract of
A. aspera
foliage against Cx.
vishnui
group
Mortality rate ( Mean ± SE)
Larval instars
Concentrations %
24 h
48 h
72 h
0.1
26.68 ± 0.94
33.32±0.94
40.00± 0.82
0.2
36.00 ± 1.63
42.62±2.05
44.00± 2.94
0.3
54.68 ± 2.05
58.68±1.70
62.68± 0.94
0.4
72.00 ± 1.63
74.68 ±0.94
77.32±1.25
First
0.5
78.68 ± 1.70
81.32 ±0.94
84.00 ± 0.82
0.1
24.00 ± 0.82
32.00± 0.82
36.00± 0.82
0.2
34.68 ± 1.25
40.00± 1.63
42.68± 2.87
0.3
52.00 ± 2.16
56.00± 1.41
60.00± 0.82
0.4
69.32 ± 1.25
72.00± 0.81
73.32± 1.25
Second
0.5
76.00 ± 1.41
77.32± 0.47
76.68 ± 0.47
0.1
22.68± 0.94
29.32± 0.47
34.68± 0.47
0.2
32.00± 0.82
37.32±1.25
40.00±2.45
0.3
49.32 ± 1.70
53.32± 1.25
57.32± 0.94
0.4
66.68 ± 0.94
69.32± 1.25
74.68± 0.47
Third
0.5
17.68 ± 0.94
73.32± 0.94
78.68± 0.47
0.1
0.00±0.00
8.00± 0.00
24.00± 0.82
0.2
2.68±0.47
9.32± 0.47
30.68± 0.47
0.3
4.00 ±0.00
13.32± 1.25
34.68± 0.47
0.4
5.32 ± 0.47
17.13± 0.47
36.00± 0.82
Fourth
0.5
6.68± 0.47
21.32± 0.47
40.00± 0.82
The larval mortality of 3
rd
instars using all the different
solvent was presented in Table 2. Acetone extract was
found to be the best larvicide, through several trials,
among all the solvent extracts of
A. aspera
leaves. The
larval mortality of
Cx. vishnui
group using acetone
extractives was depicted in Table 3 regarding all the
instars, differential time exposure and concentration
gradient as three random variables. Highest mortality
was achieved at 100 ppm of concentration
.
The LC
50
and LC
90
values of acetone extract were determined
through log-probit and regression analyses and
presented in Table 4. The larvicidal effect of acetone
extractives against
Cx. vishnui
group
were found to
be statistically significant when the mortality rate (Y)
Journal of Mosquito Research