Basic HTML Version
Journal of Mosquito Research, 2013, Vol.3, No.2, 11
-
13
ISSN 1927-646X
http://jmr.sophiapublisher.com
12
25~30
℃
and 60%~80% relative humidity. A control set
was also kept beside with same amount of distilled
water and supplementary feeding and 1
st
instar mosquito
larvae without addition of any amino acids.
3. Result and Discussion
It was found in preliminary experiments that mosquito
larvae were sensitive to amino acids. The effects of
individual amino acids on the duration of developmental
stages were presented in Table 1. The data showed that
Alanine, Glutamic acid, Phenyl Alanine and Isoleucine
have a growth retardation effect in comparison to control
set. On the other hand, Leucine, Tyrosine, Methionine
and Glycine accelerate the growth in compareson to
control set. However, growth acceleration or retardation
effect was found as density independent event.
Table 1 Developmental period analysis of amino acids to
Culex quinquefasciatus
larva
Development period (days) [Mean ± Standard Error]
Amino acids
used
Concentrations
(%)
1
st
to 2
nd
instar
2
nd
to 3
rd
instar
3
rd
to 4
th
instar
4
th
instar
to pupa
Pupa to
adult
Total
developmental
period (days)
2
3.6±0.509
5.4±0.678
4.4±0.244 4.8±0.374
2.2±0.2
20.4
3
2.6±0.244
4.2±0.374
4.6±0.244 4.6±0.509
2.6±0.244
18.6
Alanine
4
4.2±0.663
6.8±0.860
5.8±0.374 5.4±0.244
2.8±0.2
25
2
2.6±0.244
8±0.316
6.6±0.244 5.6±0.4
2.4±0.244
25.2
3
2.4±0.244
7.6±0.509
5.6±0.509 6.2±0.374
3±0.316
24.8
Isoleucine
4
3.4±0.244
8±0.447
6.2±0.374 7.2±0.374
3.2±0.374
28
2
1.2±0.2
5±0.31
5.6±0.244 4±0.316
2.6±0.244
18.4
3
1.2±0.2
5.4±0.509
4.8±0.583 3.8±0.374
3.2±0.2
18.4
Leucine
4
1.6±0.244
6.2±0.374
5.6±0.509 4.6±0.509
3.4±0.244
21.4
2
3.2±0.374
6.6±0.678
6.4±0.244 6.2±0.374
3.2±0.2
25.4
3
2.4±0.244
5.8±0.916
5.8±0.583 6±0.447
3.4±0.244
23.4
Glutamic
acid
4
3.4±0.244
5.6±0.4
5.4±0.244 6.2±0.374
3±0.316
23.6
2
1.2±0.2
4.6±0.4
4.4±0.678 5.4±0.509
3±0.316
18.6
3
1.4±0.244
4.4±0.6
5.4±0.6
3.6±0.4
3±0.316
17.8
Tyrosine
4
1.8±0.2
5.6±0.6
4.6±0.871 4.4±0.927
3.4±0.244
19.8
2
2.2±0.2
4.2±0.663
6±0.447
6±0.447
2.6±0.244
21
3
2.2±0.374
4.2±0.583
5.6±0.4
6±0.547
2.6±0.244
20.6
Methonine
4
2.6±0.244
5.2±0.734
6±0.447 4.4±0.6
2.6±0.244
20.8
2
1.2±0.2
4.4±0.748
4.8±0.489 5.2±0.583
3±0.316
18.6
3
1.2±0.2
4.2±0.583
4.8±0.583 5±0.632
3.2±0.2
18.4
Glycine
4
1.4±0.244
5.4±0.244
5.8±0.374 6.4±0.244
2.8±0.374
21.8
2
1.2±0.2
5±0.447
6±0.547 4.2±0.374
3±0.316
19.4
3
2±0.447
4.6±0.509
6.2±0.860 4.2±0.374
2.6±0.244
19.6
Phenyl
alanine
4
2.2±0.2
6.2±0.374
7±0.707 5.4±0.509
2.4±0.244
23.2
Control
3.33±0.3
8.33±0.66
5.33±0.3
6±0
3±0
21.9
Aromatic amino acids were previously documented to
play an important role in various stages of insect deve-
loppment including egg hatching factor of mosquitoes,
insecticides or repellents, production of toxicity and
feeding and food retention activities (Caroci and Nor-
iega, 2003). Present investigation provided first hand