Page 12 - IJA-Vol.3-No.17
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International Journal of Aquaculture, 2013, Vol.3, No.17, 92
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100
99
medium containing 5% human blood. Pure cultures
of bacterial isolates were spot inoculated onto blood
agar plates and β haemolytic activity was recorded
as clear zone around the colonies after incubation at
37
℃
for 24 h.
3.3
Antimicrobial Susceptibility Test
Susceptibility to antimicrobial agents was performed
for the identified
Aeromonas
spp. by the disc diffusion
method (Bauer et al., 1966). The antibiotics used and
the concentrations tested
include amoxycillin (30
mcg), carbenicillin (100 mcg), cefpodoxime (10mcg),
ceftazidime (30 mcg), cephalothin (30 mcg),
chloramphenicol (10 mcg), ciprofloxacin (5 mcg),
gentamicin (10 mcg), nalidixic acid (30 mcg),
nitrofurantoin (100 mcg), streptomycin (10 mcg),
sulphafurazole (300 mcg), tetracycline (30 mcg) and
trimethoprim (5 mcg).
Pure cultures of
Aeromonas
were inoculated into
nutrient broth and incubated at 37
℃
for 6 h-8 h. The
cultures were then seeded onto Mueller Hinton agar
plates. Antibiotic discs were placed on the surface of
the agar with sterile forceps and pressed down gently
to ensure even contact. Zone of inhibition around the
antibiotic discs was measured after 16 h-18 h
incubation at 37
℃
and susceptibility/resistance
interpretation was performed according to the
manufacturer’s interpretative table supplied by the
Hi-media laboratories, Bombay as a matching criteria.
Authors' contributions
N.J.
contributed considerably during collection, analysis and interpretation
of data, analysis of the results and write-up of the manuscript. A.A.M.H.
have made substantial contributions to the design and acquisition of data,
analysis and interpretation of results and have been involved in revising the
manuscript critically for important intellectual content; and have given final
approval of the version to be published. Both the authors read and approved
the final manuscript.
Acknowledgements
First author is thankful to Cochin University of Science and Technology for
awarding research fellowship. Authors are also thankful to the Department
of Marine Biology, Microbiology and Biochemistry for providing the
facilities to carry out the research.
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