Molecular Plant Breeding 2016, Vol.7, No.20, 1
-
8
7
4 Materials and Methods
This experiment was carried out at Holetta Agricultural Research Center during the growing season of 2014. Sixty
four breeding lines (S6) were grown in 8x8 simple lattice designs with two replications. These selfed lines were
derived from various crosses and cultivars of oilseed Brassica (Table 6). Oilseed Brassica lines other than
Ethiopian mustard (Brassica carinata) lines were introduced from abroad for breeding purpose. Each line was
planted in a plot consisting of 6 rows of 3 m length and 30 cm between rows. Data were recorded on ten randomly
selected plants from central 4 rows for plant height, number of primary branches/plant, number of secondary
branches/plant, number of pods/plant and 1 000 seed weight. Days to flowering, days to maturity and seed yield
were recorded on plot basis. All the recommended agronomic practices were followed for good establishment of
the crop. The relative efficiency of simple lattice design over randomized complete block design (RCBD) was less
than 25%, therefore, analysis of variance (Table 1) for the characters under study considered the RCBD model as
suggested by Bolanŏs and Edmeades (1996). Analysis of variance, mean performance, cluster, and principal
component were done using SAS software version 9.00 (SAS, 2002). Phenotypic and genotypic coefficients o f
variation were calculated following Burton and De Vane (1953). Broad sense heritability of the characters was
computed as suggested by Allard (1960), and the expected genetic advance under selection assuming selection
intensity of 5% (2.063) was determined following Johnson et al. (1955).
Table 6 Sources of breeding lines of oilseed Brassica
Breeding lines code ≠
Source
1,58
S67 X BRA-1030/79-436
8,9,10,11,12,13,34,38,40,43
YD X BRA-1030/79-436
5,6,7,50,56
S67 X 34477 Pakistan
2,3,4,37,42
S67 X BRA-1030/79-328
14,15,39,44
YD X BAR-1030/79-328
16,17,18
BAR-1030/79-436 X BRA-1029/79-436
22,23,24,25,26,27,28,29,30,31,32,35,36,41,45,48,49,53,54,57,59,60
S-67 X Holetta-1
19,20,46,47,52
S67 X YD
51
S67 X BRA-1029/79-436
33,55,63
YDSPS
61,64
LCSPS
21
S-67 X 509
62
Holetta-1SPS
Authors’ contribution
The first author has done the analysis and interpretation of data as well as the manuscript draft while the second author has made
substantial contributions to conception, design and acquisition of data as well as revision of the final manuscript.
Acknowledgments
Authors are grateful for Highland Oil Crops Commodity Research technical staffs for their relentless effort in collecting data for this
experiment.
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