Molecular Plant Breeding, 2016, Vol.7, No.15, 1-12
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Table 3 Micropropagation Media (MPM) for Dalbergia sissoo Roxb.
Micropropagation Media for
Shisham (MPMS)
M.S Salt
Sucrose
BAP
IAA
Gellen Gum Powder
MPMS 1
4.33 g/L
30 g/L
6.66µM 1.43 µM 2.66 g/L
MPMS 2
4.33 g/L
30 g/L
8.8 µM
1.43 µM 2.66 g/L
MPMS 3
4.33 g/L
30 g/L
11.1 µM 1.43 µM 2.66 g/L
MPMS 4
4.33 g/L
30 g/L
13.32µM 1.43 µM 2.66 g/L
MPMS 5
4.33 g/L
30 g/L
6.6 µM
1.71 µM 2.66 g/L
MPMS 6
4.33 g/L
30 g/L
8.8 µM
1.71 µM 2.66 g/L
MPMS 7
4.33 g/L
30 g/L
11.1 µM 1.71 µM 2.66 g/L
MPMS 8
4.33 g/L
30 g/L
13.32µM 1.71 µM 2.66 g/L
Table 4 Root induction Media (RIM) for Dalbergia sissoo Roxb.
Root Induction Media for
Shisham (RIMS)
M.S Salt
Sucrose
IBA
IAA
Myoinositol
Gellan Gum Powder
RM 1
2.165 g/L 30 g/L 4.9 µM 5.7 µM 100 mg/L
2.66 g/L
RM 2 (reported) used as
control
2.165 g/L 30 g/L 7.35 µM 0
0
2.66 g/L
RM 3
2.165 g/L 30 g/L 11.0 µM 0
0
2.66 g/L
3.4 Data collection and Statistical analysis
Data of micropropagation were collected in the form
of number of shoots per explant. Analysis of Variance
table was made among different media combinations
and explant sources. Experiment was designed under
CRD in factorial design and data was collected and
analyzed (Steel and Torrey, 1982).
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