International Journal of Marine Science 2016, Vol.6, No.4, 1-12
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Figure 4
Relative expression level of selected genes in 4-t-OP exposed Tg(fil-1:EGFP) zebrafish embryos were determined by real
time PCR at 48 and 72 hpf. The expressions of the cardiovascular-related genes Nkx2.5, Nkx2.7, Hand2, Tbx2a, Tbx2b, Tbx5a,
FGF1a, GATA-4, -5 and -6 was quantified. The data of the control group at 48 hpf was set as 1. The results represented triplicated
experiments, and the different letters were considered significantly at p < 0.05.