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International Journal of Marine Science 2015, Vol.5, No.9, 1-4
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4
showed that the sample preserved with Lugol’s
solution offers better accuracy in quantifying with
lower standard deviation compare to sample preserved
in mixture of formaldehyde and glutardialdehyde.
This is because Lugol’s stains cells a dark brown
colour, making counting easier and accurate (Modigh
and Castaldo, 2005). The most important is Lugol’s
solution is relatively less toxic compared to
aldehyde-based fixatives. However, iodine breaks
down easily in sunlight and samples preserved in
Lugol’s solution do not have a long shelf life (3-6
months) (Gifford and Caron, 2000). The limitation of
Lugol’s solution can be overcome easily by storing the
samples and stock solution in dark and ensure the
sample always have a “tea” like colour by adding
Lugol’s solution periodically.
4 Conclusion
It is always necessary to preserve the sample with
suitable preservative that can ensure the samples in
good condition before analyzing. Cold Lugol’s
solution is highly recommended to be used as a safe
alternative to formaldehyde-base preservative in
preserving stomach content of bivalve. Cold Lugol’s
solution not only has comparable preservative
performance to the mixture of formaldehyde and
glutardialdehyde, but it also makes the counting easier
and precise as it stains the phytoplankton dark brown
colour. Stomach content preservation studies using
different concentrations of alcohol should be
undertaken in future.
Acknowledgement
This work was supported by the project NRGS0003, a grant
from the Ministry of Higher Education Malaysia to University
Malaysia Sabah.
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