| Research Article | 5 |
| Abstract | 5 |
| Keywords | 5 |
| Introduction | 5 |
| 1 Materials and Methods | 6 |
| 2 Results | 8 |
| 3 Discussion | 12 |
| 4 Conclusion | 15 |
| Authors contribution | 15 |
| Acknowledgments | 15 |
| References | 15 |
| Figure 1 | 7 |
| Table 1 | 8 |
| Figure 2 | 9 |
| Figure 3 | 9 |
| Figure 4 | 10 |
| Table 2 | 10 |
| Table 3 | 11 |
| Table 4 | 12 |