International Journal of Marine Science 2016, Vol.6, No.22, 1-14
        
        
        
          3
        
        
          2.1.2 Korangi creek
        
        
          Korangi Creek (mangrove forest) is situated in the south of Karachi. It is covered by muddy creeks (Plate 2). The
        
        
          north most creeks of Indus delta are Korangi Creek. A Korangi Creek is 12 km from Karachi harbor and 9 km
        
        
          from Quidabad. Korangi Creek is connected at its northeast remain with Phitti Creek and Kadiro Creek while at
        
        
          its sub western end it connects with open sea and with Gizri Creek and is bounded on its sides by extensive
        
        
          mangrove vegetation of
        
        
          Avicenna marina
        
        
          (Saher, 2008). This creek receives effluents from domestic, industrial
        
        
          and oil Refinery. Its domestic, wastes comes from adjoining fishing villages (Waguder, Ibrahim Haydri, and town
        
        
          ship of Korangi). Malir River is another source of domestic and industrial wastes to Creek area. This area receives
        
        
          discharges from Pakistan Refinery, soda ash factory and National Refinery as well as Karachi Electric Supply
        
        
          Corporation Power Plant etc. (Abbas, 2006).
        
        
          Plate 2. Burrowing activity of Ocypodid crabs at Korangi Creek.
        
        
          2.1.3 Field Work
        
        
          The samples of Ocypodid crabs
        
        
          D. blanfordi
        
        
          were collected on monthly intervals for the period of March 2007 to
        
        
          May 2008 from supra tidal zone of  Clifton and Korangi Creek (Plate 1 and 2). Sampling of crabs was
        
        
          determined by quadrate method at low tide (Snowden et al., 1991; Suzuki, 1983). Each crab found within quadrate
        
        
          was captured by digging from the sand and mud up to 10cm depth from hand and with the help of forceps.
        
        
          2.1.4 Laboratory procedures
        
        
          All crabs were sorted by sieving of sand and mud (0.250 and 1.00 mm mesh size) then washed with tap water and
        
        
          placed first in 5% formalin for 24 hours and then transferred in 70% alcohol. The species and sexes were
        
        
          identified with the help of authentic available literature (Tirmizi and Ghani, 1988; Crane, 1975; Ng et al., 2008).