International Journal of Marine Science, 2016, Vol.6, No.13, 1-4
2
Figure 1 Sampling Sites, Garapan Rivermouth (left) and Cibumgur Rivermouth (right)
Sampling and Grouping
Anadara indica
Anadara indica
were collected using garoo. After that, samples were sorted based on their shell length into small
(<31.80 mm), medium (31.80-38.90 mm), and large (>38.90 mm). Sample then stored for further analysis.
Amino Acid Measurement
Content of amino acid in cockles were measured using High Performance Liquid Chromatography in Saraswanti
Indo Genetech (SIG) Laboratory. All amino acid were analyzed based on Association of Official Analytical
Chemists (AOAC) (2005).
Amino Acid standard solution. 40 µl standard was mixed with amino acid, then added with 40 µl AABA standard
internal and 920 µl aquabidest. Solution then homogenized and from this, took 10 µl standard solution. After that,
it was added sequentially with 70 µl Acc-Q-Flour Borate, vortexed and added with 20 µl Flour A reagent and
vortexed again, then allowed to stand for 1 minute, incubated for 10 minutes at 55
o
C then injected to HPLC.
Sample solution. 0.1 gr sample was added with 5 ml HCl, then vortexed. After that, solution were hydrolized for
22 hours at 110
o
C, wait until it is cool, then poured into 50 ml volumetric flask, added with aquabidest until exact
mark. Filtrate then pipetted for 500 µl into vial, then added with 40 µm AABA and 460 µl aquabidest. After
samples have been filtered and took for 10 µl standard, then added it with 70 µl Acc-Q-Flour Borate and vortexed.
It was added 20 µl flour A reagent and vortexed again, then allowed to stand for 1 minute. Incubation was done
for 10 minutes at 55
o
C, as soon as it finish then injected to HPLC
Statistical Analysis
Amino acid content both essential and nonessential in three body size (large, medium, small) of
Anadara indica
at
two location were analysed using Anova 1 Factor. The analysis was performed using statistical software.
Result and Discussion
It is known that bivalve contain high protein beside their cheap price (Srimariana et al. 2014). According to Babu
et al. (2012),
Gafrarium tumidum
contained amino acid as much as 42.97%, composed of 20.77 % esential amino
acid, and 22.2 % nonessential amino acid.
In this research, 18 amino acids were measured, which were 10 essential amino acids and 8 nonessential amino
acids. According Kirk and Othmer (1953) in Nurhayati et al. (2011), when amino acids produced between 18 up to
20 amino acids, it meant that the hydrolysis went well. The content of essential amino acid was shown at Table 1.
According to Table 1, arginin has highest concentration followed by lysin and leucine. Concentration of arginin was
high in small
A. indica
at Cibungur (1.86) and large
A.indica
at Garapan (1.47). Histidine has low concentration
compared to other amino acids, except for typtophan in small
A. indica
at Cibungur. Totally, high content of essential
amino acids were foumd in small
A. indica
at Cibungur and large
A. indica
at Garapan rivermouth.
Sampling Area at Garapan
Sampling Area at Cibungur
