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International Journal of Marine Science 2014, Vol.4, No.52, 1-9
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8
less than that of the mean MN frequency. Therefore
the present study showed that the extent of differences
in genotoxic effects, the measured as MN and BN
frequencies of the whole body tissues may be
associated with the physical and chemical properties
of the chemicals and also by other environmental
factors such as temperature, pH salinity etc (Bolognesi
and Fenech, 2012).
Therefore, it is believable that elevated concentrations
of heavy metals could have contributed to the elevated
MN frequencies in
M. phillippinarum.
The present
study concludes that the acute toxicity data can be
utilized for deriving water quality criteria and the MN
test in the mussel yields sensitive results in pollution
monitoring; especially the heavy metal pollution and
thus, it might be used as a standard method for regular
pollution monitoring of the coastal ecosystem since
MN frequency provides a useful index of accumulated
genetic damage during the lifespan of the cells and it
is an index of integrated response to the complex
mixture of contaminants monitored or not that
contributes to the genotoxic impact (Bolognesi and
Hayashi, 2011).
Authors' contributions:
CMR: Conception and participated in its design and
coordination, analysis and interpretation of data and drafted the
manuscript. MYB: Carried out laboratory toxicity tests. LP:
Carried out Micronuclei assays, done statistical application and
involved in drafting the manuscript. TM: Carried out laboratory
toxicity tests and involved in drafting the manuscript. AKK:
Conception and participated in its design and coordination and
helped drafted the manuscript.
Acknowledgement
We sincerely acknowledge the receipt of partial financial
assistance from the Ministry of Earth Sciences–ICMAM
Project Directorate, Government of India, Chennai, Tamilnadu,
India, the Department of Science and Technology
(DST-PURSE), New Delhi and Ministry of Environment and
Forests (MoEF), New Delhi to carry out the research work.
References
Aguilera A., Manrubia S.C., Gómez F., Rodríguez N. and Amils R., 2006,
Eukaryotic Community Distribution and Its Relationship to Water
Physicochemical Parameters in an Extreme Acidic Environment, Río
Tinto (South-western Spain), Applied Environmental Microbiology, 72:
5325-5330
Alink G.M., Quik J.T., Penders E.J., Spenkelink A., Rotteveel S.G., Maas
J.L., and Hoogenboezem W. 2007, Genotoxic effects in the Eastern
mudminnow (
Umbra pygmaea
L.) after exposure to Rhine water, as
assessed by use of the SCE and Comet assays: a comparison between
1978 and 2005, Mutation Research, 631: 93-100
APHA-AWWA-WPCF., 1995, Standard Methods for the Examination of
Water and Wastewater, 19
th
edn. American Public Health Association,
New York
Arslan O.C., Parlak H., Katalay S., Boyacioglu M., Karaaslan M.A., and
Guner H., 2010, Detecting micronuclei frequency in some aquatic
organisms for monitoring pollution of Izmir Bay (western Turkey),
Environmental Monitoring and Assessment, 165: 55-66
ASTM, 1990, Standard guide for conducting 10-day static sediment toxicity
tests with marine and estuarine amphipods, American Society for
Testing and Materials, Philadelphia, 1367-1390
ASTM Standard E729, 2007. Standard Guide for Conducting Acute Toxicity
Tests on Test Materials with Fishes, Macroinvertebrates, and
Amphibians, ASTM International, West Conshohocken, PA, DOI:
10.1520/E0729-96R07
Barsiene J., Lazutka J., Syvokiene J., Dedonyte V., Rybakovas A., Bagdonas
E., Bjornstad A., and Andersen O.K., 2004, Analysis of micronuclei in
blue mussels and fish from the Baltic and the North Seas,
Environmental Toxicology, 19: 365-371
Bolognesi C., and Fenech M., 2012, Mussel micronucleus cytome assay,
Nature Protocol, 7: 1125-1137
Bolognesi C., and Hayashi M., 2011, Micronucleus assay in aquatic animals.
Mutagenesis, 26: 205-213
Bolognesi C., Frenzilli G., Lasagna C., Perrone E., and Roggieri P., 2004,
Genotoxicity biomarkers in
Mytilus galloprovincialis
: wild versus
caged mussels. Mutation Research, 552: 153-162
Bolognesi C., Landini E., Roggieri P., Fabbri R., and Viarengo A., 1999,
Genotoxicity biomarkers in the assessment of heavy metal effects in
mussels: experimental studies. Environmental and Molecular
Mutagenesis, 33: 287-292
Bolognesi C., Perrone E., Roggieri P., and Sciutto A., 2006. Bioindicators in
monitoring long term genotoxic impact of spill: Haven case study.
Marine Environmental Resesarch, 65: S287-S291
Bonacker D., Stoiber T., Bohm K.J., Prots I., Wang M., Unger E., Their R.,
Bolt H.M., and Degen G.H., 2005, Genotoxicity of inorganic lead salts
and disturbance of microtubule function, Environmental and Molecular
Mutagenesis, 45(4): 346-353
Braunbeck T., Boettcher M., Hollert H., Kosmehl T., Lammer E., Leist E.,
Rudolf M., and Seitz N., 2005, Towards an alternative for the acute fish
LC
50
test in chemical assessment: the fish embryo toxicity test goes
multi-species- an update, Altex
,
22: 87-102
Burgeot T., His E., and Galgani F., 1995, The micronucleus assay in
Crassostrea gigas
for the detection of seawater genotoxicity, Mutation
Research, 342: 125-140
Camargo J.A., and Alonso A., 2006, Ecological and toxicological effects of
inorganic nitrogen pollution in aquatic ecosystems: A global
assessment, Environment International
,
32: 831-849
Cavas T.C., and Ergene-Gözükara S., 2003, Micronuclei, nuclear lesions
and interphase silver stained nucleolar organizer regions (AgNORs) as
cyto-genotoxicity indicators in
Oreochromis niloticus
exposed to
textile mill effluent, Mutation Research, 534: 93-99
Cavas T.C., and Ergene-Gozukara S., 2005, Induction of micronuclei and
nuclear abnormalities in
Oreochromis niloticus
following exposure to