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International Journal of Marine Science 2014, Vol.4, No.50, 1-22
http://ijms.biopublisher.ca
22
Table A4. Microsatellite analysis of host corals from the variable temperature experiment (VTE)
. Seven microsatellite loci were assessed in order to genotype the 12
Seriatopora hystrix
colonies (6 from the upwelling site [UWS; i.e., Houbihu] and 6 from the non-upwelling site [NUWS; i.e., Houwan]) of the VTE. Additional details about the microsatellites (e.g., fluorescent
labeling of the respective primers) can be found in the manuscripts in which they were first published (Maier et al., 2001; 2005; 2009; Underwood et al., 2006). “F” and “R” are abbreviations
for forward and reverse, respectively.
Locus
NCBI accession
number
Primer sequences (‘5-3’)
Motif
Annealing
temperature (
℃
)
Sh4-001
DQ131572
F: ACTTCATTCAATTATCCATAAAGTCAT
(CATT)
10
61
R: ATCACTGATTAGACTTACAAACAAACTG
Sh2.15
AY604005
F: CGTGCCACTGTGATTTCTTC
(CA)
18
60
R: AACAAAAACGTCTCCATTACCC
Sh2-002
DQ131573
F: GTGAATAAGAACGACGGA
(CA)
18
60
R: AAATACTAATTACAGGCATGAC
Sh4.28
AF320769
F: TGTGGTCTACAGTATATCTTTTGTG
(CA)
11
61
R: CAAATTTGAATCTACAGTGGGG
Sh2-006
DQ131577
F: CAAATACCAATCAGTGTAGCA
(CT)
8
, TT, (CT)
7
61
R: GGCCTAATATCTGTCTCCTTC
Sh4.24
AY604006
F: TCCTCCAGATGAATTTGAACG
(CA)
37
61
R: TTCAGGGAAGATTTGCCG
Sh3-004
DQ131575
F: GGTTACTTATGTGTTTTGCTT
(AAC)
12
53
R: AGTTGATGTTGTTGCCTT