Basic HTML Version
International Journal of Marine Science 2014, Vol.4, No.50, 1-22
http://ijms.biopublisher.ca
6
Table 2 Host coral genotyping results.
“N” = sample size. “N
A
” = number of alleles. “N
PA
” = number of private alleles. “H
o
” =
observed heterozygosity. “H
e
” = expected heterozygosity. “F
IS
” = inbreeding coefficient. “UWS” = upwelling site (Houbihu).
“NUWS” = non-upwelling site (Houwan).
Locus
UWS
NUWS
Allele size range (base pairs)
N
6
6
Sh4-001
N
A
2
1
126-184 (UWS), 184 (NUWS)
N
PA
1
0
H
o
0.17
0
H
e
0.17
0
F
IS
0.00
NA
Sh2.15
N
A
3
4
268-277 (UWS), 268-278 (NUWS)
N
PA
0
1
H
o
0.17
0.50
H
e
0.71
0.71
F
IS
0.78*
0.32
Sh2-002
N
A
4
5
123-146 (UWS), 123-146 (NUWS)
N
PA
1
2
H
o
0.50
0.83
H
e
0.77
0.76
F
IS
0.38
-0.11
Sh4.28
N
A
5
4
106-119 (UWS), 107-117 (NUWS)
N
PA
2
1
H
o
0.67
0.67
H
e
0.74
0.64
F
IS
0.11
-0.053
Sh2-006
N
A
3
3
105-114 (UWS), 105-110 (NUWS)
N
PA
1
1
H
o
0.50
0.50
H
e
0.44
0.44
F
IS
-0.15
-0.15
Sh4.24
N
A
6
7
260-315 (UWS), 259-313 (NUWS)
N
PA
4
5
H
o
0.83
0.50
H
e
0.88
0.89
F
IS
0.057
0.46*
Sh3-004
N
A
3
2
148-154 (UWS), 148-151 (NUWS)
N
PA
1
0
H
o
0.50
0.50
H
e
0.59
0.41
F
IS
0.17
-0.25
Overall
Mean
N
A
3.7
4.2
3.9
N
PA
0.48
0.58
0.53
H
o
0.62
0.64
0.63
H
e
0.24*
0.099
0.16*
Notes:
*
p
<0.05.
1.6 Site of origin comparisons
Upon pooling data across experiments (Figure 6), the
Symbiodinium
GCP (data not shown) was found to be
similar between sites of origin (student’s
t
-test,
t
=0.91,
p
=0.37). On the other hand,
rbcL
expression was
significantly higher (16-fold) in samples from the
UWS relative to those of the NUWS (Wilcoxon
Z
=3.5,
p
<0.001). Although expression of
psI
was statistically
similar between the two sites (
Z
=1.3,
p
=0.19),
pgpase
was expressed at 2.0-fold higher levels in corals of the
UWS (
Z
=4.7,
p
<0.001). In contrast,
nrt2
was expressed
at similar levels across sites (student’s
t
=0.74,
p
=0.46), as was
apx1
(Wilcoxon
Z
=1.8,
p
=0.076).
Finally,
Symbiodinium hsp70
was expressed at signi-
ficantly higher (4.1-fold) levels in corals from the
NUWS relative to those of the UWS (
Z
=3.6,
p
<0.001).