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International Journal of Marine Science 2013, Vol.3, No.40, 319-332
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329
gelatinase production, acid production from
carbohydrates) as described by Ruangpan and Kitao
(1991). Additional biochemical tests were performed
as per standard methods and compared with standard
reactions for confirmation (Alsina and Blanch, 1994;
Holt et al., 1994). Species level identification was
carried out wherever possible using the Bergey’s
Manual of Determinative Bacteriology (Holt et al.,
1994).
3.6 Food conversion ratio and average survival of
shrimps
Weight (g) of thirty shrimps collected from each pond
was recorded at fortnightly intervals from 30-doc
onwards. The following calculations were carried out:
Food conversion ratio (FCR) = total feed used in the
culture pond / net production of shrimps
Average survival (%) = (total no. of shrimps survived /
total no. of post-larvae stocked)
× 100
3.7 Statistical analysis
Temporal and spatial fluctuations in the variables
(bacteriological and environmental variables) were
assessed by Analysis of Variance (ANOVA) with time
(days of culture) and space (ponds and/or sources
water and/or with stations) as sources of variation.
Because the variances were not homogenous and/or
the residual departed from normality as indicated by
Cochran's test, all the bacteriological data were
transformed into log
10
(x+1). Student’s
t
-test was
performed to detect significant differences in bacterial
counts between shrimp pond components. The degree
of correlation between abiotic and biotic variables was
analyzed by means of Pearson’s correlation for rearing
water and sediment samples. All statistical analyses
were performed using PC based Minitab statistical
package (Minitab Inc., USA).
Authors' contributions
PAL and RAS conceived the study. RAS, SSK, SVK and RRS participated
in the sampling and sample analysis. SOF analyzed the data and drafted the
manuscript.
Acknowledgements
We thank the Director, National Institute of Oceanography, Goa (India) for
the facilities. The present study was funded by the Indo-Norwegian
Programme for Institutional Co-operation, New Delhi. This is NIO
contribution no. 5440.
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