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International Journal of Marine Science 2013, Vol.3, No.37, 295-305
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304
extraction for the isolation and estimation of these
forms rather the total collagen, the quantification
procedures are same to deduce the collagen quantity in
all the sponges. As observed in the present study,
collagen content is higher in
Callyspongia,
explanation to this may lie in the presence of high
collagenous material in the heavily packaged
proteinaceous sheets in the sponge tissue. Based on
the present data, the potential candidate for the
characterization of this collagen and collagenous
nature will be verified further by various biophysical
and molecular techniques.
The precipitated collagen is completely soluble in the
cold acid and salt extraction buffers. However, more
percentage of the precipitated collagenous material
remains insoluble, which gives the Ins collagen
content of the sponge tissue of all the four sponges. It
is understood from the present results that the high
percentage of the Ins form of the collagen in the
sponges are much responsible for the tissue integrity
and unique skeletal framework of the individual
sponges, this fact may be responsible for the presence
of high Ins collagen than AS and SS collagen forms.
Acknowledgement
The authors are thankful to the Task Force Project (GAP 0276) of
Department of Biotechnology (DBT), Ministry of Science and Technology,
Government of India for financial assistance, and also thankful to the
Director, IICT for providing the facilities and constant encouragement.
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