Basic HTML Version
International Journal of Marine Science 2013, Vol.3, No.26, 201-211
http://ijms.sophiapublisher.com
207
Identification and quantification of 2,3,7,8-substituted
congeners of PCDDs/DFs and dioxin-like PCBs (non-
and mono-ortho-substituted congeners) was performed
by use of a (i) Shimadzu GC-14B gas chromatograph
with AOC-1400 auto-sampler. Columns: CBP-1
(SE-30) and CBP-5 (SE-52/54 confirmatory column).
Injection: Splitless (30 seg.) 300
℃
. Temperature
program of the oven: 110
℃
(1 min.); 15
℃
/min up
170
℃
; 7.5
℃
/min up to 290
℃
, hold for 10 minutes.
Total run time: 25 minutes. Electron Capture Detector
(
63
Ni) temperature: 310
℃
; (ii) HPLC: Shimadzu
LC-10AS; Mobile phase: acetonitrile: water 80%,
isocratic run. Column: Shimadzu STR-ODS-II (C-18
reverse phase) 25 cm, L: 4mm ID. UV/VIS detector
model: Shimadzu SPD-10A.
The results of chemical analysis for the compounds
analyzed and the initial data are expressed as (pg/g,
w.w.) and (pg TEQ/g, w.w.). Where the concentration
of a specific compounds remains below the detection
limits (nd), this was treated as a zero value. Toxic
equivalent factors (TEQ), recommended by WHO,
were used for the further analysis of the data (Table 4).
TEQ of the analyzed PCDD/Fs and dioxin-like PCBs
(DL-PCBs) were calculated using the WHO-TEF
values (Van Den Berg, 2006). TEQ is the product of
the concentration of an individual dioxin-like
compound in an environmental mixture and the
corresponding TCDD-TEF for that compound.
Table 4 Dioxin content in the fish factors of toxic equivalent factors of thereof (TEF)
Dioxin-like PCBS, PCDDS, and PCDFS
Toxic Equivalent factor
Polychlorinated dibenzodioxins (PCDD)
2,3,7,8
-
tetrachlorodibenzodioxin (TCDD)
1
1,2,3,7,8
-
pentachlorodibenzodioxin
1
1,2,3,4,7,8
-
hexachlorodibenzodioxin (HxCDD)
0.1
1,2,3,6,7,8
-
hexachlorodibenzodioxin (HxCDD)
0.1
1,2,3,7,8,9
-
hexachlorodibenzodioxin (HxCDD)
0.1
1,2,3,4,6,7,8
-
heptachlorodibenzodioxin (HpCDD)
0.01
Octa
-
chlorodibenzodioxin (OCDD)
0.0001
Polychlorinated dibenzofurans (PCDF)
2,3,7,8
-
tetrachlorodibenzofuran (TCDF)
0.1
1,2,3,7,8
-
pentachlorodibenzofuran (PeCDF)
0.05
2,3,4,7,8
-
pentachlorodibenzofuran (PeCDF)
0.5
1,2,3,4,7,8
-
hexachlorodibenzofuran (HxCDF)
0.1
1,2,3,6,7,8
-
hexachlorodibenzofuran (HxCDF)
0.1
1,2,3,7,8,9
-
hexachlorodibenzofuran (HxCDF)
0.1
2,3,4,6,7,8
-
hexachlorodibenzofuran (HxCDF)
0.1
1,2,3,4,6,7,8
-
heptachlorodibenzofuran (HpCDF)
0.01
1,2,3,4,7,8,9
-
heptachlorodibenzofuran (HpCDF)
0.01
Octachlorodibenzofuran (OCDF)
0.0001
Non-orto PCB
3,3’,4,4’
-
tetrachlorobiphenyl (PCB 77)
0.0001
3,4,4’,5
-
tetrachlorobiphenyl (PCB 81)
0.0001
3,3’,4,4’,5
-
pentachlorobiphenyl (PCB 126)
0.1
3,3’,4,4’,5,5’
-
hexachlorobiphenyl (PCB 169)
0.01
Mono-orto PCB
2,3,3’,4,4’
-
pentachlorobiphenyl (PCB 105)
0.0001
2,3,4,4’,5
-
pentachlorobiphenyl (PCB 114)
0.0005
2,3’,4,4’,5
-
pentachlorobiphenyl (PCB 118)
0.0001
2’,3,4,4’,5
-
pentachlorobiphenyl (PCB 123)
0.0001
2,3,3’,4,4’,5
-
hexachlorobiphenyl (PCB 156)
0.0005
2,3,3’,4,4’,5’
-
hexachlorobiphenyl (PCB 157)
0.0005
2,3’,4,4’,5,5’
-
hexachlorobiphenyl (PCB 167)
0.00001
2,3,3’,4,4’,5,5’
-
hexachlorobiphenyl (PCB 189)
0.0001
Equation 1 is the formula for calculating exposure
concentration for n DLCs in a mixture in TCDD TEQ.
Exposure to the
i
th
individual PCDD, PCDF, or PCB
compound is expressed in terms of an equivalent
exposure of TCDD by computing the product of the
concentration of the individual compound (
Ci
) and its
assigned
TEFi
. TEQ is then calculated by summing
these products across the
n
DLCs compounds present
in the mixture. The TEQ may be compared to the
dose-response slope for TCDD and used to assess the