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International Journal of Marine Science 2013, Vol.3, No.19, 151-157
http://ijms.sophiapublisher.com
156
Table 2 Effect of the extract of
Aurora globostellata
on SRBC induced delayed type hypersensitivity (DTH) in Rat
Animal group Treatment dose (mg/kg
-1
)
DTH response (mm of mean paw edema thickness ) Time interval (h)
3 h
6 h
9 h
12 h
24 h
Group I
Control
34.1±0.24 3.50±0.04 3.70±0.15 3.80±0.26 2.10±1.20
Group II
T
1
(50mg/kg
-1
)
3.8±0.28
2.90±0.04 2.85±0.12 2.25±1.42 1.15±0.12
Group III
T
2
(150mg/kg
-1
)
3.2±0.14
2.40±0.08 21.40±0.34 1.50±1.80 1.40±1.30
Group IV
T
3
(250mg/kg
-1)
2.1±0.18
1.34±0.02 1.96±0.15 0.41±0.21 0.64±0.35
Note: Each data represents the mean ± SE of 6 Wister rats
Figure 1 Effect of the extract of
S.inconstans
var.
globosa
on
SRBC induced delayedtype hypersensitivity (DTH) in Rat
3.4 Effect of the extracts of
S. inconstans
on T- cell
and B-cell count:
The effect of three concentrations of the extract of
A.
globostellata
was tested in Rat to find out whether this
extract can influence the lymphopoeiosis (T-cell and
B-cell). In the control rat the amount of B-cell was
20±1% but in standard drug given rat, it was 26±1%
on 7
th
day 57.6±1 on 14
th
day. In immunosuppressed,
Dexamethazone treated rats it was 14±1 on 7
th
day and
15±1 on 14
th
day. In
A. globostellata
extract treated rat,
B-cell count was increased to 34±1 on 7
th
day and
23±0.6 on 14
th
day at 250mg/kg treated rat. Like
B-cell the total number of T-cell count also increased
due to the treatment with extract. When compared to
B-cells, T-cells count was high (Table 3). The extracts
of
Ag
were found to enhance T-cell and B-cell
production after their production was suppressed by
immune suppressive drug.
The results of the present study indicated that the
extracts of
Aurora globostellata
have bioactive
compounds which can stimulate both cell mediated
and humoral immune response. The hike in T-cell and
Table 3 Effect of the extract of
Aurora globostellata
and on lymphocyte count in the Rat in different duration of treatment
Animal group Treatment dose (mg/kg)
No of lymphocytes/ml blood
7
th
day
14
th
day
B cell
T cell
B cell
T cell
Group I
Control
20±1
54.2±1
20±1
53.3±1
Group II
Dexamethasone 0.5 mg/kg
26±1
58±1
24±1
57.6±1
Group III
Immun Aid (Pro immune Tablets)
14±1
42±1
15±1
39.0±1
Group IV
T
1
(50mg/kg)
28±0.8
51±0.4
62±1
59.1±1
Group V
T
2
(150mg/kg)
30±0.7
66±0.7
30±0.7
65.4±1
`Group VI
T
3
(250mg/kg
)
34±0.9
67±1.2
33±0.6
68.0±1
Note: Each data represents the mean ± SE of 6 Wister rats
B-cell population in Rats treated with the extract
showed that the extract has metabolites to enhance
lymphopoiesis in Rat. As Jensen et al
(1994) reported,
immune enhancing properties of
Ag
could be useful in
the adjuvant treatment of various diseases. Hence a
further research is in progress to find out the exact
compound that enhances T-cell and B-cell immunity.
4 Conclusion
The extracts of sponge were tested to find out whether
it can do any role in immunomodulation. Both cell
mediated immunity and antibody mediated immunity
were analyzed using murine model after administering
the sponge extracts. The extracts of sponge were
found to enhance antibody titre and B-cell count. Total
T-cells and Delayed type hypersensitive reactions