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Int. J. of Marine Science 2012, Vol.2, No.6, 43
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50
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Figure 4 Antibacterial activity of purified compounds from
marine sponge
Aurora globostellata
3.7 Sponge extracts elution
Based on the antimicrobial assays, the extract that
registered the maximum antimicrobial activity was
selected for further study. EtoAc extract was very
effective in antimicrobial properties so the ethyl
acetate extract were chosen for further separation. The
crude EtOAc extract (13.2 g) was applied over a flash
chromatography column of silica gel 60 (150 g) and
eluted with a solvent gradient system of EtOAc and
MeOH, Fractions (50 mL) being collected as follow:
The various fractions were collected from followed
solvent system: 1) Hexane 100%; 2) Hexane 99%:
ethyl acetate 1%; 3) Hexane 98%: ethyl acetate 2%; 4)
Hexane 96%: ethyl acetate 4%. Up to 22
nd
fraction
were eluted. The fractions thus obtained were once
again evaporated and concentrated. They were again
assayed for antibacterial, antifungal activity and
spectral studies.
Acknowledgements
We are grateful to Dr. A.J.A. Ranjit Singh, Principal, Sri Paramakalyani
College, Alwarkurichi, Tirunelveli, Tamilandu, India for providing the
laboratory facilities to carry out the microbiological work. We acknowledge
the
financial
support
received
from
PURSE
scheme,
Ref.No:MoES/11-MRDF/1/25/P/09-PC Ministry of Earth and Science,
Government of India, New Delhi.
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