IJMS-2017v7n42 - page 13

International Journal of Marine Science, 2017, Vol.7, No.42, 399-410

http://ijms.biopublisher.ca

404

Figure 9 Crude oil 2.0% with

Brev. dimi./vesicu.

On the other hand,

Vibrio vulnificus

showed high degradation percentage of PAH (84.23%) in concentration 2.0

mg/l and low degradation (22.91%) in concentration 0.5 mg/l (Table 2; Figure 10; Figure 11; Figure 12; Figure

13). The genus of

Vibrio

was first described as a degrading phenanthene organism from West and colleagues, who

isolated large numbers of strains by spreading Chesapeake Bay samples onto complex media each with a

superficial layer of phenanthrene (Okpokwasili et al., 1984; West et al., 1984).

Sphingomona paucimobilis

showed high degradation percentage (97.39%) in concentration 2.0 mg/l and low

degradation (27.26%) in concentration 0. 5 mg/l (Table 2; Figure 14; Figure 15; Figure 16; Figure 17). This is

consistent with a report by Tao et al. (2007) who found that this bacterium has unique genes to degrade a wide

range of PAH and related compounds. The gene is related from other Pseudomonas genera and reports so far in

sequence homology and gene organization. Also Pinyakong et al. (2003a; 2003b) observed that, many

sphingomonads degrade naphthalene, anthracene, and phenanthalene by common pathways w e r e found in

other gram-negative bacteria. In addition, many researchers (Kim et al., 1996; Feng et al., 1997; Romine et al.,

1999; Ogram et al., 2000; Basta et al., 2004) found large plasmids in strains of

Sphingomonas xenobiotic

contributing to the degradation of PAH.

Figure 10 Crude oil 0.25% with

V. vulnificus

min

FID1A,FrontSignal(FADIL2\4.D)

Acenaphthlene

Acenaphthene

Fluorene

Phenanthrene

Anthracene

Fluoranthene

Pyrene

Benzo(a)fluoranthene

Chrysene

Benzo(b)fluoranthene+Benzo(k)fluoranthene

Dibenz(a)pyrene

Indeno(1,2,3-cd)pyrene+Dibenz(a,h)anthracene

Benzo(g,h,i)perylene

min

FID1A,FrontSignal(FADIL2\5.D)