International Journal of Horticulture 2015, Vol.5, No.7, 1
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Table 3 Influence of seed treatments and containers on drymatter production 10 seedling
-1
(mg) during storage
Pre storage seed treatment
(T)
Container (C) and storage periods in months (P)
Cloth bag
HDPE bag
PLAF bag
0
3
6
Mean 0
3
6
Mean 0
3
6
Mean
Control
7.0
6.2 5.8
6.3
7.0
6.3
6.0
6.4
7.0
6.4
6.2
6.5
Carbendazim @ 2g kg
-1
(A) 7.0
6.4 6.2
6.5
7.0
6.4
6.3
6.5
7.0
6.4
6.4
6.6
Imidacloprid @2ml kg
-1
(B)
7.0
6.5 6.4
6.6
7.0
6.7
6.6
6.7
7.0
6.8
6.8
6.8
(A) + (B)
7.0
6.7 6.6
6.7
7.0
6.9
6.8
6.9
7.0
7.0
6.8
6.9
Mean
7.0
6.4 6.2
6.5
7.0
6.5
6.6
6.6
7.0
6.6
6.5
6.7
(T X P)
Control
7.0
6.3 6.0
6.4
Level of significance
Carbendazim @ 2g kg
-1
(A) 7.0
6.4 6.3
6.5
P
C
T
P X C PX T T X C PX CxT
Imidacloprid @2ml kg
-1
(B)
7.0
6.6 6.6
6.7
SEd
0.040 0.040 0.046 0.069 0.080 0.080 0.138
(A) + (B)
7.0
6.8 6.7
6.8
CD (P=0.05) 0.079 0.079 0.091 0.137 0.158 NS
NS
Mean
7.0
6.5 6.4
6.6
Seed deterioration alters the semi-permeability
properties of the membrane and membrane integrity
(Berjack and Villiers, 1972). Electrical conductivity of
seed leachate increased gradually over periods of
storage (0.075 dSm -1 to 0.139 dSm -1 ) irrespective of
seed treatments and containers (Table 4). The
probable reason for increase in the electrical
conductivity might be due to loss of membrane
integrity (Copeland, 1988). The increase in
conductivity of electrolites was slow in Carbendazim +
Imidochlorprid treatment that were stored in
aluminium foil pouches while it was higher in the
seeds of cloth bag. The Carbendazim +
Imidochlorprid @ 2 g kg
–1
seed, + 100 mg kg
–1
seed
formulations had antibacterial and antifungal
properties besides stabilizing the week carbon bonds
of unsaturated fatty acids and helped in the integrity of
cell membrane. This was clearly exhibited by the
lowest electrical conductivity (0.075 dSm -1 ) recorded
by Carbendazim + Imidochlorprid treated seeds. This
was in accordance with the earlier reports of Ramya
(2003) reported the superiority of aluminium foil
pouch containers in storage of tomato seeds. Ramesh
and Ukey (2006) observed that tomato seed treated
with Imidacloprid @ 5, 7.5 and 10 g kg
-1
seed helped
to control the white fly and also increased the seed
germination and survival of seedling. With increased
storage period, the germination, vigour and protein
content decreased (Table 5) while the electrical
conductivity increased irrespective of treatments and
storage containers. This may be probably due to
increase moisture content of the seeds (Mukherjee,
2001) depletion of food reserves (Kovalenko, Badev
and Falik, 1977), changes in protein structure
(Abdul-Baki and Anderson, 1973), loss in membrane
integrity and increased incidence of storage fungi.
Table 4 Influence of seed treatments and containers on electrical coductivity (dSm
-1
) during storage
Pre storage seed
treatment (T)
Container (C) and storage periods in months (P)
Cloth bag
HDPE bag
PLAF bag
0
3
6
Mean 0
3
6
Mean 0
3
6
Mean
Control
0.075 0.147 0.225 0.149 0.075
0.135 0.214 0.141 0.075 0.130 0.210 0.138
Carbendazim@2g kg
-1
(A) 0.075 0.130 0.221 0.142 0.075
0.121 0.222 0.139 0.075 0.118 0.205 0132
Imidacloprid@2ml kg
-1
(B)
0.075 0.136 0.218 0.143 0.075
0.120 0.229 0.141 0.075 0.117 0.202 0.131
(A) + (B)
0.075 0.138 0.215 0.142 0.075
0.117 0.216 0.136 0.075 0.115 0.204 0.131
Mean
0.075 0.137 0.219 0.144 0.075
0.123 0.220 0.139 0.075 0.120 0.205 0.133
(T X P)
Control
0.075 0.137 0.217 0.145 Level of significance
Carbendazim@2g kg
-1
(A) 0.075 0.122 0.216 0.139
P
C
T
P X C PX T T X C PX CxT
Imidacloprid @2ml kg
-1
(B)
0.075 0.124 0.216 0.138 SEd
0.000 0.000 0001 0.001 0.001 0.001 0.003
(A) + (B)
0.075 0.123 0.211 0.136 CD (P=0.05) 0.001 0.001 0.002 0.003 0.003 NS
0.006
Mean
0.075 0.127 0.215 0.139
