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International Journal of Horticulture 2014, Vol.4, No.17, 1
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The infested seeds were set aside in a plastic container
(4.2 x 9.0cm) and covered with muslin cloth till the
emergence of adult. Healthy adults that emerged from
the initial culture were transferred to another plastic
container (4.2 x 9.0cm) and provided with clean
uninfested cowpea seeds for oviposition and
maintained in the laboratory. Prior to insect infestation
the beans were sterilized in Gallenkamp oven to avoid
contamination and overlapping of generations
(Allotey and Azalekor, 2000). The container was
undisturbed until the emergence of adults. Freshly
emerged subsequent generations were used for the
experiments.
Assessment of insecticidal properties of powder of
Hyptis suaveolens
(l.)
Ten freshly emerged adults of
C. maculatus
were
introduced into the white disposable plastic container
(4.2 x 9.0cm) already containing 20g of cowpea
treated with 0.05g, 0.1g, 0.2g, 0.3g and 0g (control)
of
H. suaveolens
. Mortality of the cowpea beetles
were made at 24hours, 48hours and 72hours.
Percentage adult’s mortality was corrected using
Abbot (1925) formula.
After seven days of oviposition, numbers of egg laid
on treated seed were recorded; number of seeds with
eggs and without eggs was also counted. The
experiment was laid out in a Completely Randomized
Design with each treatment replicated three times.
After the data on oviposition parameter have been
taken, experimental set-up was kept undisturbed till
the emergence of adults. The numbers of adults that
emerged from the seeds 21days after infestation with
C. maculatus
were recorded. Data were also obtained
on seeds with holes, weight loss after adult emergence.
Germination tests were also carried out on the seeds
treated with various concentrations of
H. suaveolens
after a month of storage. The germination test was
done by arranging ten seeds in a petridish (8.5 x
1.2cm) lined with moistened facial tissue paper.
Counting was done after 5days of planting.
Data analysis
Data on number of egg laid, number of seeds with
eggs and without eggs, number of seeds with holes
and without holes, adult emergence were square root
transformed while data on germination test was
arcsine transformed before subjecting to the analysis
of variance (ANOVA) using SPSS statistical package
version 15.0. Means were separated using Duncan
multiple range test at 95% level of significance.
Acknowledgement
The authors are thankful to Mr. Mr. G. F. Hassan, a technologist
in the department for the provision of the hyptis leaf. They also
appreciate Mrs. Adekanle for her advice and technical support
during the study.
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