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International Journal of Horticulture 2014, Vol.4, No.10, 50
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52
http://ijh.biopublisher.ca
52
linkage block. The apparent linkage of an additional
unnamed gene from the suggested that the
Ur-3
region
may contain an even greater complex of linked genes
than
Ur-3
alone. Similar finding were reported by
Pastor and Corrales (2003).
In cross involving IC-525236, all the plants in F
1
’s
exhibited polymorphism at 620 bp which indicated
that the resistance to rust is dominant in nature in
IC-525236. In F
2
progeny of cross IIHRPB-7×
IC-525236, out of 100 plants, 71 plants showed
polymorphic DNA at 620 bp for SK 14, remaining 29
plants did not exhibit polymorphism
.
χ
2
values also
indicated that the ratio for observed to expected was
not significant. These results indicated that the
segregation was in the pattern of Mendelian ratio of
3:1 which confirmed that the inheritance to rust
resistance is monogenic dominant (Aghora et al., 2007;
2008). Therefore, it would be easy to transfer the
resistant gene to any desirable background. Thus,
resistance can be easily incorporated with any other
commercial cultivars through back cross breeding
using resistant parent as donor. These findings are in
accordance with Yuebin (1993); Sayler et al. (1995)
and Aghora et al. (2007; 2008).
Material and Methods
Experiments on breeding pole type french beans
(
Phaseolus vulgaris
L.) for resistance to rust using
molecular markers were carried out at Division of
Vegetables crops and Division of Biotechnology,
Indian Institute of Horticultural Research, Bengaluru,
2012 to 2013. Experimental material consisted of resistant
parent, IC525236 and three susceptible pole type good
pod quality lines namely, IIHRPB-1, IIHRPB-2 and
IIHRPB-7. Crossing was carried out by emasculation
and hand pollination between resistant and susceptible
parents to develop F
1
and F
2
populations. DNA of
these genotypes were extracted using CTAB method.
The present study aims with three SCAR markers
namely, SK 14, SA 14 and SBC 6 used as foreground
selection to integrate the rust resistant genes into genetic
background of susceptible or elite cultivar (Table 3).
Amplification of DNA from parents, F
1
’s and F
2
’s was
carried out. Each PCR programme was carried out at
different annealing temperatures as shown in the Table 3.
Table 3 SCAR markers linked to rust (
Uromyces phaseoli
) resistance used in present study
No. SCAR name Marker of origin Size(bp)/Orientation
Sequence of SCARs F: 5′ to
3′, R: 5′ to 3′
Tagged Locus Linkage group
1 SK 14
K14
1079/800
Codominant
F:CTA TCT GCC ATT ATC
AAC TCAAAC
R:GTG CTG GGAAAC ATT
ACC TAT T
Ur-4
B6
2 SA 14
A14
620 cis
F:CCC GCT ACA CAC CAA
TAC CTG
R:CCC GCT ACA CTT GAT
AAAATG TTA G
Ur-3
B11
3 SBC6
BC06
308 cis
GAA GGC GAG AAG AAA
AAG AAAAAT
GAA GGC GAG AGC ACC
TAG CTG AAG
Ur-6
11*
Authors' contributions
BD: Participated in carrying out the entire work as M. Sc research program;
TSA: Participated in the designing of the study and acted as chairperson to
carry out the work under his guide lines; NM: Helped in collecting
germplasms and carrying out hybridization work and to study the
inheritance pattern in French bean crosses; HPS: Helped to draft the
manuscript and participated in the sequence alignment
References
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Reben Wint) in French bean (
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