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International Journal of Horticulture 2014, Vol.4, No.10, 50
-
52
http://ijh.biopublisher.ca
50
Research Report
Open Access
Breeding Pole Type French Bean (
Phaseolus vulgaris
) for Rust (
Uromyces
phaseoli
Reben Wint) Resistance using Molecular Markers
B. Divya
1,
, T. S. Aghora
2,
, N. Mohan
2,
, D.C. Lakshman Reddy
2,
and C. Aswath
2,
1. University of Horticultural Sciences, PG Center, Bengaluru-560065, India
2. Indian Institute of Horticultural Research, Bengaluru- 560 089, India
* Corresponding author email:
aghor@iihr.ernet.in
Authors
International Journal of Horticulture, 2014, Vol.4, No.10 doi: 10.5376/ijh.2014.04.0010
Received: 14 Apr., 2014
Accepted: 21 Apr., 2014
Published: 07 May, 2014
Copyright
© 2014 Divya
et al
., This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Divya
et al.
, 2014, Breeding Pole Type French Bean (
Phaseolus vulgaris
) for Rust (
Uromyces phaseoli
Reben Wint) Resistance using Molecular Markers,
International Journal of Horticulture, 2014, Vol.4, No.10 50-52 (doi: 10.5376/ijh.2014.04.0010)
Abstract
French bean (
Phaseolus vulgaris
L.) 2n=14 is an important legume vegetable grown for its tender green pods which are
rich in proteins, minerals and dietary fibre. Though it is a short duration crop, it is prone to several biotic and abiotic stresses. Among
the biotic stresses, rust caused by
Uromyces phaseoli
(
Reben Wint) has become epidemic in bean growing areas and the yield loss to
the tune of 80% is reported. Rust fungi have wide variability in pathogenicity and also demonstrate frequent sexual recombination
and more than 150 physiological races of the pathogen are reported. Hence, the induction of genetic resistance is always preferred
because it is least expensive and has no adverse environmental effects. Inheritance of rust resistance was studied in F
2
generation by
using molecular markers. Resistance was confirmed in parent genotype IC525236 by using SK 14. In IC525236 resistance to rust is
controlled by single dominant gene.
Keywords
French bean; Rust resistance; Single dominant gene; SK 14
Introduction
French bean,
Phaseolus vulgaris
L. (2n=22) is a
member of the family Fabaceae. It is an important
legume vegetable grown for its tender green pods
either for fresh consumption or for processing In
world, french bean is grown over an area of 1.48
million ha with annual production of 17.65 million
MT and the productivity of 11.95 t/ha. In India, its
cultivation is in 0.22 million ha with production of
0.68 million MT and productivity of 2.8 t/ha (1). Like
any other crops, french bean is also susceptible to
various biotic and abiotic stresses. Among the biotic
stresses, rust (
Uromyces phaseoli
Reben Wint.) has
become epidemic in bean growing areas. The yield
loss due to rust ranges from 18% to 78% (2 and 3).
Recently, the trend in french bean cultivation is
changing from bush type to pole type beans because
of their long yielding period and high yield. Popular
pole bean varieties like NZ, NZ Super King, US-2,
HAPB-4 etc, though high yielding, they are all
susceptible to rust disease causing heavy loss to
farmers. Thus, the present study was under taken to
incorporate rust resistance into quality french bean
cultivars using molecular markers (SCAR) linked to
rust resistance.
Result and Discussion
Confirmation of rust resistance in parents
Four parental genotypes
viz
., IC52236, IIHRPB-1,
IIHRPB-2 and IIHRPB-7 were used for confirmation
of rust resistance in french bean using validated
SCAR markers
viz.,
SK 14. Initially, genomic As the
banding pattern was intact in all the genotypes, primer
SK 14 was used to study the polymorphism for rust
resistance. SK14 showed resistant banding pattern at
620 bp (Table 1; Figure 1).
Table 1 Estimates for rust resistance in several crosses of F
1
population
Observed frequency
Expected frequency
No. F
1
genotypes
Generations
Resistant
Susceptible
Resistant
Susceptible
Total
1 IIHRPB-1
Susceptible Parent
0
90
0
90
90
2 IIHRPB-2
Susceptible parent
0
88
0
88
88
3 IIHRPB-7
Susceptible Parent
0
84
0
84
84
4 IC-525236
Resistant Parent
85
0
85
0
85
5 IIHRPB-1×IC-525236 F
1
100
0
100
0
100
6 IIHRPB-2×IC-525236 F
1
98
0
98
0
98
7 IIHRPB-7×IC-525236 F
1
81
0
81
0
81