International Journal of Horticulture, 2017, Vol.7, No.16, 133-137
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2.2 Culture media for in vitro micropropagation
Eight different media were formulated for
in vitro
micropropagation of cv. PRI-Red. Each medium contains basal
MS salt and vitamins, supplemented with 0, 0.75, 1.75, 2.75 mg/l NAA and 0, 0.75, 1.75, 2.75 mg/l BAP, alone or
in combination. All media components were mixed together, adjusted to pH 5.7-5.8 and solidified with 2.66 g/l
Gellun gum powder. Thereafter, these media were autoclaved at 121˚C and 15
psi
for 20 minutes.
2.3 Micropropagated shoots induction
Axillary buds (explant) were cultured on all eight media described, kept at 26±1˚C for 16/8 hrs light/dark regimes
and observed daily. After 5d, buds started germination or/shoot induction. Subsequent maintenance of these
cultures was achieved by biweekly subcultures.
3 Results and Discussion
When sprouts of 0.3% GA3 treated potato tubers (Figure 1) of cv. PRI-Red were used as explants and were
cultured on micropropagation media containing 1.75, 2.75 mg/l BAP, then these so called berries like structures
were formed (Figure 2). These berries like structures were of different shapes and sizes (Figure 3). Similarly
when calli of cv. Kuroda induced on callus induction medium containing 4.5 mg/l 2-4D were when shifted on
regeneration medium containing 4.75 mg/l BAP then same so-called berries like structures were formed (Figure
4). These so-called berries like structure were started sprouting ~2 months after formation. Shoots were started to
grow from these so- called berries (Figure 5).
Figure 1 Sprouts of potato tubers of cv. PRI-Red treated with GA
3
