International Journal of Horticulture, 2017, Vol.7, No. 6, 40-46
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these media. RM1 medium showed 32% regeneration efficiency and most of the explants just increased in size
and showed callogenesis instead of direct
in vitro
regeneration. RM
2
medium having kinetin and IAA 1 mg/L and
0.25 mg/L respectively showed highest regeneration efficiency (94%), where bunch of shoots (4.733
shoots/explant) emerged from every explant within 8 weeks of culturing (Table 1; Table 2; Figure 1). RM
3
medium having kinetin and IAA in 1 mg/L and 0.75 mg/L respectively showed 16% regeneration efficiency and
only a few explants regenerated into shoots with an average of 0.8 shoots / explant. In present study, RM
4
medium
having kinetin and IAA in 2 mg/L and 0.125 mg/L respectively showed 30% regeneration efficiency with 1.53
shoots / explant. RM
5
medium showed only 6% regeneration efficiency with very poor response to regeneration.
RM
6
medium showed 44% regeneration efficiency with 2.2 shoots / explant (Table 1; Table 2; Figure 1).
Table 1 Analysis of Variance for Leaf explants of
N. plumbaginifolia
Source
DF
SS
MS
F
P
Treatment
5
36.6244
7.32489
2.27
0.1141
Error
12
38.8000
3.23333
Total
17
75.4244
Table 2 LSD All-Pairwise Comparisons of Data for Explants
Treatment
Mean
Homogeneous Groups
RM2
4.7333
A
RM6
2.2000
AB
RM1
1.6000
AB
RM4
1.5333
B
RM3
0.8000
B
RM5
0.2667
B
Figure 1
In vitro
proliferation and hardening of regenerated Leaf explant on RM2 regeneration medium
Note: A, B. Showed regeneration after 4 weeks of culturing C, D, E. Close-up of individual explant showing emergence of multiple
shoots/explant. F. Regenerants shifted to jars for rooting G. Accilimitaziation
In present study, RM
2
showed highest regeneration efficiency where the concentration of kinetin and IAA was 1
mg/L and 0.25 mg/L respectively. On the other hand RM
5
medium having 2 mg/L, 0.25 mg/L kinetin and IAA
respectively exhibited minimum regeneration efficiency. The study revealed optimal level of IAA and Kinetin
required for efficient direct regeneration of
N. plumbaginifolia
. This indicated the effect of PGRs on regeneration
