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Genomics and Applied Biology 2015, Vol. 6, No. 2, 1-7
http://gab.biopublisher.ca
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Figure 3 Gene Ontology Result
it shows that, within the Molecular Function category,
genes encoding binding proteins and proteins related
to catalytic activity were the most enriched. Proteins
related to metabolic processes and cellular processes
were enriched in the Biological Process category. With
regard to the Cellular Components category, the cell
and cell part were the most highly represented
categories.
A total of 500 unigenes were annotated with 122
pathways
in
the
KEGG
database
(http://www.genome.jp/kegg/pathway.html).
Many
transcripts include various pathways like metabolic
pathways, plant-pathogen interaction pathways, fatty
acid metabolism pathway and fatty acid biosynthesis.
Figure 4 WEGO Tool Result
4. SSR mining
Microsatellite markers (SSR markers) are some of the
most successful molecular markers in the construction
of a
Vicia sativa
L. genetic map and in diversity
analysis (Zhang et al). For identification of SSRs, all
transcripts were searched with perl script MISA. We
identified a total of 1150 SSRs in 1055 transcripts
(Table 5). The mono-nucleotide SSRs represented the
largest fraction of SSRs identified followed by
tri-nucleotide and di-nucleotide SSRs. Although only
