Genomics and Applied Biology 2019, Vol.10, No.1, 1-9
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The predicted target genes were involved in different biological processes, including transcription factors, signal
transduction, stress response and so on. There were 70 hypothetical transcription factors in all target genes,
divided into 22 families (Table 3), mainly involved in signal transduction and regulation of gene expression. The
target genes of miR156 had squamosa promoter-bindingprotein-like (SPL) family transcription factors, which was
the most transcription factor family obtained in this research. The target gene of miR164 had NAC transcription
factor family, and MYB transcription factor was regulated by miR319, miR845, miR156a and new miRNA.
Transcription factors, such as bHLH and C3H, were predicted to be regulated by new miRNA in Zijuan tea.
Table 3 Transcription factors of miRNA target genes
miRNA name
Target genes
Classification
Annotation
novel_122
c131734_g1
AP2-EREBP
Ethylene-responsive transcription factor WIN1
vvi-miR393b
c133787_g3
AP2-EREBP
Ethylene-responsive transcription factor ERF095
novel_131
c143355_g1
AP2-EREBP
Ethylene-responsive transcription factor 1B
vvi-miR172d
c145738_g2
AP2-EREBP
Floral homeotic protein APETALA 2
vvi-miR167c
c145911_g2
ARF
Auxin response factor 8
vvi-miR167c
c142995_g1
ARF
Auxin response factor 6
vvi-miR160c
c105552_g2
ARF
Auxin response factor 22
vvi-miR160c
c138760_g3
ARF
Auxin response factor 18
vvi-miR160c
c144604_g2
ARF
Auxin response factor 18
novel_65
c128190_g2
ARID
AT-rich interactive domain-containing protein 2
novel_65
c136889_g1
ARR
Two-component response regulator ARR12
novel_65
c121580_g1
bHLH
Transcription factor bHLH96
novel_115
c145849_g5
bHLH
Transcription factor bHLH63
novel_28
c142451_g1
bHLH
Transcription factor bHLH140
novel_65
c140877_g1
bHLH
Transcription factor bHLH112
novel_122
c120785_g1
bHLH
Putative transcription factor bHLH041
vvi-miR166a
c147128_g3
HD-ZIP
Homeobox-leucine zipper protein ATHB-15
novel_65
c143290_g5
C2C2-Dof
Dof zinc finger protein DOF2.4
vvi-miR156a
c142141_g3
C2C2-Dof
Dof zinc finger protein DOF1.4
novel_22
c147552_g4
C3H
Zinc finger CCCH domain-containing protein 53
vvi-miR396b
c141369_g1
C3H
Zinc finger CCCH domain-containing protein 45
novel_113
c138573_g1
C3H
Zinc finger CCCH domain-containing protein 32
novel_36
c144893_g1
C3H
Zinc finger CCCH domain-containing protein 30
novel_65
c116662_g2
C3H
Zinc finger CCCH domain-containing protein 19
novel_14
c146236_g3
C3H
Zinc finger CCCH domain-containing protein 18
novel_65
c133527_g1
C3H
Zinc finger CCCH domain-containing protein 1
novel_118
c143725_g1
FAR1
Protein FAR1-RELATED SEQUENCE 7
novel_14
c128321_g1
FAR1
Protein FAR1-RELATED SEQUENCE 6
novel_65
c129367_g1
FAR1
Protein FAR1-RELATED SEQUENCE 5
vvi-miR172c
c144198_g1
FAR1
Protein FAR1-RELATED SEQUENCE 3
novel_35
c120645_g1
FAR1
Protein FAR1-RELATED SEQUENCE 1
vvi-miR166a
c138612_g2
GRF
Growth-regulating factor 5
novel_65
c129141_g1
HMG
High mobility group B protein 3
novel_54
c129692_g1
HMG
High mobility group B protein 13
vvi-miR167a
c104063_g1
LIM
LIM domain-containing protein WLIM2b
vvi-miR156b
c138027_g1
LIM
LIM domain-containing protein WLIM1
novel_115
c98901_g1
LOB
LOB domain-containing protein 16
novel_115
c147951_g1
LOB
LOB domain-containing protein 1
novel_118
c133888_g2
MADS
MADS-box protein SVP
novel_65
c135878_g1
MYB
Putative Myb family transcription factor
novel_14
c137136_g2
MYB
Myb-related protein 306
vvi-miR156a
c113937_g1
MYB
Myb family transcription factor APL
novel_65
c11831_g1
MYB
Myb-related protein Myb4
