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Computational Molecular Biology 2014, Vol. 4, No. 14, 1-9
http://cmb.biopublisher.ca
2
(
japonica
),
Arabidopsis thaliana
(
Mouse-ear cress
),
CKX3 of
Oryza Sativa
(
japonica
),
Arabidopsis
thaliana(Mouse-ear cress)
, CKX4 of
Oryza Sativa
(
japonica
), CKX5 of
Arabidopsis thaliana(Mouse-ear
cress)
, CKX6 of
Arabidopsis thaliana
(
Mouse-ear cress
)
and CKX7 of
Arabidopsis thaliana
(
Mouse-ear cress
)
and CKX8 of
Oryza Sativa
(indica).The main objective
of the study is to find out their physic-chemical
properties, similarities and differences among all the
enzymes. However the function of all the eznzymes is
same, but their properties are explained below.
Material and Methods
The cytokinin dehydrogenase enzymes of rice, wheat
and maize were selected in this study due to its role in
high grain production in crops.
Sequence retrieval
The cytokinin dehydrogenase enzymes of
O.sativa
(japonica), A.thaliana (mouse-ear cress
) and
zea mays
(maize)
were used for this study. Their amino acid
sequences were retrived from uniprot (http://www.
uniprot.org/), which is given in Table 1.
Table 1 The informations of all enzymes which were collected from Uniprot
species
UniprotID Function
Length of amino acids
o.sativa (indica)
A2XNV3
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
532
o.sativa (japonica)
Q4ADV8
Catalyzes the oxidation of cytokinin, modulates the number of
reproductive organs by regulating the cytokinin accumulation in
inflorescence meristem, major QTL involved in high grain
production
565
o.sativa (japonica)
Q5JLP4
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
529
o.sativa (japonica)
Q8LNV6
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
527
A.thaliana
(mouse-ear cress)
Q9FUJ1
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
524
A.thaliana
(mouse-ear cress)
Q9FUJ3
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
501
A.thaliana
(mouse-ear cress)
Q67YU0
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
540
Zea mays
Q9T0N8
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group. Cleaves zeatin, isopentenyladenine,
isopentenyladenosine, zeatinriboside and cis-zeatin, but not
dihydrozeatin, kinetin and benzylaminopurine.
534
A.thaliana
(mouse-ear cress)
Q9LY71
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenylgrou
533
A.thaliana
(mouse-ear cress)
Q9LTS3
Catalyzes the oxidation of cytokinins, a family of N(6)-substituted
adenine derivatives that are plant hormones, where the substituent is
an isopentenyl group
523