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Computational Molecular Biology 2014, Vol. 4, No. 12, 1-8
http://cmb.biopublisher.ca
8
Figure 11 Gradient PCR with
Apo1
gene specific primer,
Apo1-1 (promoter) 1 - HGN1 (high grain), 2- Heera( low grain)
Annealing Temperatures are given on the top of gel
3 Conclusions
As mentioned above the main objective was to find
out the functional, non-functional regions of high
grain number genes along with the comparative
structural analysis of their respective proteins. Three
deigned primers for
Dep1
gene, one for
Ghd1
gene and
one for
Apo1
gene were tested for amplification from
high and low grain varieties, HGN1 and Heera,
respectively. Two primers, Dep1-2 (promotor) for
Dep1
gene and Ghd7-2(Exon1) for
Ghd7
gene
amplified fragments from high grain variety, HGN1
while failed to amplify from low grain variety, Heera,
indicating that these primers would be useful for
introgressing genes from high grain varieties into low
grain varieties to improve yield potential through
molecular breeding approach in future.
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