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Computational Molecular Biology 2014, Vol. 4, No. 11 1-13
http://cmb.biopublisher.ca
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Figure 12 Result page of ConSeq Tool
Figure 13 Result page of ConSeq Tool when no conserved sequence found
2.3.3 RNA-Seq Data analysis
De novo means assembling short reads to create
full-length (sometimes novel) sequences.
De novo sequencing involves sequencing a novel
genome for the first time, and requires specialized
assembly of sequencing reads. The unique
combination of read length, read depth, and flexible
paired-end insert sizes makes Illumina sequencers
ideal for de novo sequencing. Unparalleled raw read
accuracy enables confident and efficient production of
high quality, long contig assemblies. Data of both
Illumina and Roche 454 are analyzed with five
different species.
The detailed analyses of the data set has provided