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Computational Molecular Biology
2014, Vol.4, No.6, 1-5 http://cmb.biopublisher.ca
Research Report
Open Access
Phylogeny in Few Species of Leguminosae Family Based on
matK
Sequence
Sagar S. Patel , Dipti B. Shah
G. H. Patel Post Graduate Department of Computer Science and Technology, Sardar Patel University, Vallabh Vidyanagar, Gujarat-388120, India
Corresponding Author email:
sgr308@gmail.com
;
Computational Molecular Biology, 2014, Vol.4, No.6 doi: 10.5376/cmb.2014.04.0006
Copyright
© 2014 Patel and Shah. This is an open access article published under the terms of the Creative Commons Attribution License, which permits
unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In this paper, few species of Leguminosae family considered for phylogenetically analyses which are found in Gujarat
state in India and
matK
gene sequence data from NCBI database are considered for evolutionary analysis. The sequence data of the
matK
gene are more accurate than rbcL sequence data in the reconstruction of phylogenies throughout the seed plants. Leguminosae
family is one of the largest families that contain thousands of species of Plants, Herbs, Shrubs and Trees worldwide. This study
shows that species of Leguminosae family which is further classified into Fabaceae (Papilionaceae), Mimosaceae and
Caesalpiniaceae; based on morphological characters has different members and the based on the DNA and protein
matK
sequence
data analysis, few species are not related with each other as per morphological classification. We conclude that few species are
related with each other as per botanical or morphological classification of Leguminosae family but evolutionary results shows that
based on DNA and protein
matK
sequence data some species are not related with morphological or taxonomical classification.
Keywords
Leguminosae family; Bioinformatics; NCBI;
matK
1 Introduction
Leguminosae family contains species of Plants, Herbs,
Shrubs, and Trees. Legumes are used as crops, forages
and green manures; they also synthesize a wide range
of natural products such as flavours, drugs, poisons
and dyes. The legume family is the third largest family
of angiosperms (Mabberley, 1997) with approximately
730 genera and over 19,400 species worldwide (Lewis
et al., in press). Legumes are able to convert
atmospheric nitrogen into nitrogenous compounds
useful to plants. This is achieved by the presence of
root nodules containing bacteria of the genus
Rhizobium. These bacteria have a symbiotic
relationship with Legumes, fixing free nitrogen for the
plants; in return legumes supply the bacteria with a
source of fixed carbon produced by photosynthesis.
The predilection of legumes for semi-arid to arid
habitats is related to a nitrogen-demanding metabolism,
which is thought to be an adaptation to climatically
variable or unpredictable habitats whereby leaves can be
produced economically and opportunistically (McKey,
1994), (Wojciechowski et al). Leguminosae family is
further classified into three subfamilies; Fabaceae
(Papilionaceae), Caesalpiniaceae and Mimosaceae
(http://en.wikipedia.org).
1.1
matK
gene
The
matK
gene, formerly known as orfK, is emerging
as yet another gene with potential contributions to
plant molecular systematics and evolution (Johnson
and Soltis, 1994, 1995; Steele and Vilgalys, 1994;
Liang and Hilu, 1996; Gadek, Wilson, and Quinn, in
press). The gene, ~1500 base pairs (bp), is located
within the intron of the chloroplast gene
trnK
, on the
large single-copy section adjacent to the inverted
repeat (Figure 1). Further, the molecular information
generated from
matK
has been used to resolve
phylogenetic relationships from shallow to deep
taxonomic levels (Johnson and Soltis, 1994;
Hayashi and Kawano, 2000; Hilu et al., 2003;
Cameron, 2005).
Preferred citation for this article:
Patel and Shah, 2014, Phylogeny in Few Species of Leguminosae Family Based on
matK
Sequence, Computational Molecular Biology, Vol.4, No.6 1-5 (doi:
10.5376/cmb.2014.04.0006)
Received: 11 Feb., 2014
|
Accepted: 10 May, 2014
|
Published: 17 Jul., 2014