Animal Molecular Breeding 2015, Vol. 5, No. 1, 1-8
7
current study (OPB04) generated interesting results on
genetic diversity and population differentiation of
A.
marginata
, however all the three (3) primers used could
not amplify the genomic DNA from one of the samples
(that is, A11), perhaps on account of the presence of
polyphenolic compounds or polysaccharide in the
DNA of the said sample.
In conclusion, the genetic and morphometric analysis
of 16 accessions of
A. marginata
collected from three
(3) different locations in southern Nigeria using
RAPD-PCR and average linkage cluster analysis
revealed high level of genetic diversity and
heterogeneity. Of the 84 scorable bands that were
generated in the RAPD analysis using OPB03, OPB04,
and OPB08 primers, 79 of the bands (accounting for
94.04% of the total number of bands) were
polymorphic. The OPB04 primer gave 30 RAPD
fragments exhibiting significant genetic differentiation
across all samples. At the population level, this primer
revealed significant genetic differences between the
samples from Cross River State (albino-bodied form)
and each of the remaining samples from Akwa Ibom
and Edo States (black), respectively. This study is the
first to elucidate genetic diversity in African land snail
species that are prevalent in Nigeria. Given that the
RAPD markers used in this study showed genetic
variability among a small sample of
A. marginata
found in the southern part of the country, it would
mean that with the use of greater resolution markers
including single nucleotide polymorphisms (SNPs)
and simple sequence repeat markers (SSRs) on a
greater number of diverse samples, the full range of
diversity in African land snail accessions in Nigeria
could be determined.
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