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3.5 Statistical Methods
Using ITS sequence of
Pachysandra
terminalis
as a
reference, the number of nucleotide substitutions was
counted out based on the principle of using pair-wise
comparison. Using one parameter model of Jukes and
Cantor (1969) and two-parameter model of Kimura
(1968) calculated the base substitution data.
Authors’ Contributions
XFW and NL were the persons who conducted experimental
design and performances and XFW also finished the data
analysis and wrote the manuscript. KSJ was principal investi-
gator who conceived the project and supervised the experimental
designing, data analyzing, and paper writing and revising. All
authors have read and agreed the final context of manuscript.
Acknowledgement
This study was funded by the “Eleventh Five-Year” National
Science and Technology Support Project (NO.2006BAD01A1403).
The authors thanked Zhong- shan Botanical Garden of Chinese
Academy of Sciences for providing plant materials.
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