Triticeae Genomics and Genetics 2015, Vol.6, No.2, 1-7
5
Table 3 The values of each different pattern for 12 bread wheat genotypes by using SDS-PAGE marker system
S.
No
Number of bands
observed
Numberof
polymorphic bands
Polymorphism
(%)
Nei's gene
diversity (h)
Shannon's Information
index (I)
H
T
H
S
D
ST
G
st
N
m
1.
28
26
92.85
0.3662
0.5355
0.3662 0.00 0.3662 1.00 0.00
Jaccard’s similarity coefficient based SDS-PAGE
banding pattern was used for cluster analysis to study
genetic relationship. The range of genetic similarity
was found in between 0.29-1.00 (Supplementary Table
S-3). The dendogram (Figure 2) clearly indicated two
main clusters. The cluster 1 includes G1 (MP-4010),
G2 (HI-8713) and G3 (C-306). In subcluster salt
susceptible genotypes G1 (MP-4010) and G2
(HI-8713) are exactly same to each other lies at 1.00
Jaccard similarity coefficients. Cluster 2 includes G4
(Lok-1), G5 (Raj-4037), G6 (HD-2932), G7 (KH-65),
G8 (KRL-19), G9 (KRL-213), G10 (KRL-210), G11
(KRL-1-4) and G12 (Raj-3765). It has two subcluster,
in which subcluster one includes salt susceptible
genotypes G4 (Lok-1) and G5 (Raj-4037) are exactly
same with similarity coefficient 1.00. Subcluster two
includes genotypes G6 (HD-2932, G7 (KH-65), G8
(KRL-19), are also lies at 1.00 similarity coefficient.
Both clusters are related to each other at 0.56
similarity coefficient.
Figure 2 Dendogram generated for wheat genotypes using UPGMA cluster analysis based on Jaccard Similarity Coefficient
2.2 Diversity analysis
In the present study, the population diversity based on
SDS PAGE electrogram patterns was calculated using
POPGENE 1.31 software. The Shannon diversity
index (I) is one common diversity index often used to
evaluate allelelic diversity in a locus. Shannon’s index
accounts for both abundance and evenness of the
alleles present (Shannon and Weaver, 1949), and are
useful for understanding allele structure at a locus and
measures gene diversity. A close perusal of Table 3
reveals that Shannon’s information index, was found
to be 0.5355 and Nei’s (1973) gene diversity or
expected heterozygosity (H
e
) another common
diversity index in population genetics was 0.3662 and
total genetic diversity during the present study was
found to be 0.3662 and also equivalent to Nei’s gene
diversity or expected heterozygosity (H
e
). Based on
the fact that the diversity within variety was not
observed during study, shows that varieties are well
maintained to avoid any genetic contamination
through a chance of cross pollination. Total genetic
diversity (H
T
) and genetic diversity within varieties
(H
S
) were used for the determination of the
inter-variety genetic diversity (D
ST
= H
T
− H
S
).
