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Triticeae Genomics and Genetics 2012, Vol.3, No.4, 38-43
http://tgg.sophiapublisher.com
A Letter Open Access
Characrerizing the Sequences of
Kr
Gene in Different Genotypes of Common
Wheat
Hua Cai
1
Qingqing Liu
1, 2
1 School of Biology and Food Engineering, Chuzhou University, Chuzhou, 239000, P.R. China; 2 School of Biology and Basic Medical Sciences, Soochow
University, Suzhou, 215123, P.R. China
Corresponding author email: chczh@163.com;
Authors
Triticeae Genomics and Genetics, 2012, Vol.3, No.4 doi: 10.5376/tgg.2012.03.0004
Received: 16 Oct., 2012
Accepted: 29 Oct., 2012
Published: 12 Nov., 2012
Copyright: © 2012 Cai and Liu.
This article was first published in Molecular Plant Breeding (2012, 10(4): 418-422) in Chinese, and here was authorized to
translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and
reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article
:
Cai and Liu, 2012, Characrerizing the Sequences of
Kr
Gene in Different Genotypes of Common Wheat, Triticeae Genomics and Genetics, Vol.3, No.3 38-43
(doi: 10.5376/tgg.2012.03.0004)
Abstract
The incompatibility of distant hybridization in wheat has been known to be controlled by the
Kr
gene. In order to figure
out the characteristics of the
Kr
gene in different genotypes of wheat, the six genotype germplasms selected from the Chinese wheat
micro-core collection, called Mazhamai, Xiaobaimang, Tuokexunyihao, Chinese Spring, Yangmai, and Zhengmai9023, were
employed to be experimental materials for identifying the characteristic of the
Kr
genes by means of the homology cloning approach.
The results showed that the three of six genotypes could amplify a fragment of Kr gene with 414 bp in length. The sequences among
three cloned fragments shared 99.8% homology except two sites with SNP differences. Blast analysis indicated that the cloned
sequences of partial
Kr
genes have 85% homology with the S-locus receptor kinase gene in the same regions. Thus, we preliminarily
speculated the
Kr
gene might possess similar functions as the gene conferring self-incompatibility in plant, which possibly facilitated
cellulae mastoideaes to accumulate calloses in chapitersand affected the competitive ability of exogenous pollens eventually resulting
in the incompatibility of distant hybridization in wheat.
Keywords
Wheat;
Kr
gene; Distant hybridization; Plant self-incompatibility
Background
The relative genera and species of common Wheat
(
Triticum estivum
L.) bear a lot of excellent genes. By
means of distant hybridization the favorable genes of
relative plants could be transferred into wheat (Dao,
2009), which would be important significance of
enriching wheat genetic basis and breeding excellent
new variety. However, the most important issue facing
the wheat distant hybridization is the genes, the
dominant
Kr1
and
Kr2
genes (commonly call
Kr
gene), located in chromosomes of 5BL and 5AL
chromosome, which can effectively inhibit the
heterologous pollen mating with the host to decrease
hybridization affinity. So, the
Kr
gene also was known
as the gene of distant hybridization incompatibility in
wheat (Manickavelu et al., 2009b).
The studies showed that the activity of the
Kr
gene in
the different system of the distant hybridization were
very different, In the hybridization in the closer
relative species e.g. wheat×rye (
Secale cereale
L.), the
dominant
Kr
gene actively suppresses the pollen
affinity, whereas in distantly relative species e.g.
wheat×maize (
Zea mays
L.), the dominant
Kr
gene
maintains inactive (Torres et al., 2010). So far, what
the mechanism of the
Kr
gene suppresses the distant
hybridization is unclear yet.
Previously, we happened that there were significant
differences of hybridization affinity in distant
hybridization of wheat and maize, either in the crosses
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